Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
308,299 |
T→C |
54.4% |
Q30R (CAG→CGG) |
maa ← |
maltose O‑acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 308,299 | 0 | T | C | 54.4%
| 15.9
/ 63.8
| 81 | Q30R (CAG→CGG) | maa | maltose O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (14/23); new base C (20/24); total (34/47) |
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCAGCGAGAATTTGCTGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACT > minE/308231‑308353
|
gATCAGCGAGAATTTGCTGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCt < 1:2639874/69‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:2144446/68‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:599551/68‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:3325195/68‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:3424896/68‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:1508055/68‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:1780479/68‑1 (MQ=255)
tGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:1721521/68‑1 (MQ=255)
ggCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCg < 1:2097548/67‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2575175/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:57660/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2174399/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2394689/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2460302/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2507077/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2366062/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2674367/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:273641/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:285632/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3043745/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3164191/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3211920/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1059504/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1717566/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1693061/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:928397/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1956833/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1376983/69‑1 (MQ=255)
tgCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:1269587/69‑1 (MQ=255)
tgCTCTTCCGACAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:922492/69‑1 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:3023059/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2962328/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:3371364/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2809542/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:1660151/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2068655/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2577174/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:841058/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2605817/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2497661/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2067107/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2015013/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:746876/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2225595/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:555522/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:2280686/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:53849/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:1863807/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:504692/1‑49 (MQ=255)
ctctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAgctcgc > 1:393432/1‑49 (MQ=255)
ctTCCGCCAGGGAATGATTGTATCGGTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:3031295/65‑1 (MQ=255)
ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:2615585/59‑1 (MQ=255)
ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:3279468/59‑1 (MQ=255)
ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:72236/59‑1 (MQ=255)
ccGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:3215312/59‑1 (MQ=255)
gCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCa < 1:1454033/66‑1 (MQ=255)
gCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCa < 1:1814558/66‑1 (MQ=255)
ccAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTg < 1:1705059/69‑1 (MQ=255)
ccAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTg < 1:2467180/69‑1 (MQ=255)
ccAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTg < 1:3102738/69‑1 (MQ=255)
gAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGa > 1:2432989/1‑68 (MQ=255)
aTGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGc > 1:2693057/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCTAGATAACGTCTCATCTGCCGAGCGAt > 1:3129215/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGa > 1:1251056/1‑67 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGa > 1:2172645/1‑67 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:3073529/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2503483/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:145431/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2489707/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2295033/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:3171708/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2153388/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2041823/1‑68 (MQ=255)
aTTGTATCGGTGAATAAGCTGACCAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGAt > 1:2943871/1‑68 (MQ=255)
aTCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCa < 1:3001582/50‑1 (MQ=255)
tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:2277418/68‑1 (MQ=255)
tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:1421016/68‑1 (MQ=255)
tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:1713765/68‑1 (MQ=255)
tCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACt < 1:442256/68‑1 (MQ=255)
ggTGAATAAGCCGACGAGCTCGCAGGCGATCGCGAGATAAc < 1:2947601/41‑1 (MQ=255)
tGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGAt < 1:1992857/36‑1 (MQ=255)
tGAATAAGCCGACGAGCTCGCTGGCGATCGCGAGATAAc < 1:951636/39‑1 (MQ=37)
|
GATCAGCGAGAATTTGCTGGCGTAATGTGTGCTCTTCCGCCAGGGAATGATTGTATCGGTGAATAAGCTGACGAGCGCGCAGGCGATCGCGAGATAACGTCTCATCTGCCGAGCGATACAACT > minE/308231‑308353
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
