Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	317,602	A→G	51.1%	L866L (TTA→TTG)	kefA →	fused mechanosensitive channel proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	317,602	0	A	G	51.1%	6.2 / 129.5	141	L866L (TTA→TTG)	kefA	fused mechanosensitive channel proteins
Reads supporting (aligned to +/- strand): ref base A (46/23); new base G (6/66); total (52/89)
Fisher's exact test for biased strand distribution p-value = 2.63e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 141 total aligned reads displayed.

AGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGCGTCGTATGCCAT  >  minE/317537‑317658

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGGCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:2596070/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:2763358/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:866132/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:1598523/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:896417/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:3025193/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:2434555/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:3380914/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:942422/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:2460723/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:1384868/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:1355115/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:2072555/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:975950/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:2826183/69‑1 (MQ=255)

aGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:1049055/69‑1 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:1686473/68‑1 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTAc                                                         >  1:1935242/1‑66 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACt                                                        >  1:2142130/1‑67 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACt                                                        >  1:1785004/1‑67 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACt                                                        >  1:2056351/1‑67 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACt                                                        >  1:199598/1‑67 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACt                                                        >  1:1682172/1‑67 (MQ=255)

 gTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACt                                                        >  1:870020/1‑67 (MQ=255)

   cTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGaa                                                    >  1:1537146/1‑69 (MQ=255)

   cTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGaa                                                    >  1:245357/1‑69 (MQ=255)

   cTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGaa                                                    >  1:2889974/1‑69 (MQ=255)

   cTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGaa                                                    >  1:1198092/1‑69 (MQ=255)

   cTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGaa                                                    >  1:598816/1‑69 (MQ=255)

    tgttgtTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGaa                                                    >  1:677394/1‑68 (MQ=255)

    tgttgtTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAg                                                   >  1:1545630/1‑69 (MQ=255)

    tgttgtTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAg                                                   >  1:3075631/1‑69 (MQ=255)

    tgttgtTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAg                                                   >  1:1491691/1‑69 (MQ=255)

                  aTCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTg                                                       <  1:797321/51‑1 (MQ=255)

                    cGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTcgcg                                   >  1:834039/1‑69 (MQ=255)

                    cGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTcgcg                                   >  1:2329104/1‑69 (MQ=255)

                     gCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTcgcg                                   <  1:2871839/68‑1 (MQ=255)

                     gCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTcgcg                                   <  1:1862103/68‑1 (MQ=255)

                     gCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTcgcg                                   <  1:991561/68‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACt                                >  1:3349947/1‑67 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTg                               >  1:2679376/1‑68 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTg                               >  1:1417418/1‑68 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTg                               >  1:1802238/1‑68 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:1728914/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:993983/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:1161527/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:1262028/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:925631/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:673274/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:2252279/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:1905406/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:412259/69‑1 (MQ=255)

                         cAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTCCTCTCGCGACTGa                              <  1:699544/69‑1 (MQ=255)

                             tgtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:1494084/2‑69 (MQ=255)

                             ggtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:23980/1‑69 (MQ=255)

                             ggtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:2931185/1‑69 (MQ=255)

                             ggtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:795160/1‑69 (MQ=255)

                             ggtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:134138/1‑69 (MQ=255)

                             ggtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:3287807/1‑69 (MQ=255)

                             ggtggCCTGGGCGTTGATTCGCAACCTGCCTGGTATACTGGAAGTGCTGGTGctct                                       >  1:2424805/1‑56 (MQ=255)

                             ggtggCCTGGGCGTTGATTCCCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:1044244/1‑69 (MQ=255)

                              gtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAatat                          >  1:3293957/1‑68 (MQ=255)

                              gtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATg                         >  1:2744430/1‑69 (MQ=255)

                              gtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATg                         >  1:1183926/1‑69 (MQ=255)

                              gtggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATg                         >  1:1476834/1‑69 (MQ=255)

                                ggCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATgc                        >  1:2121141/1‑68 (MQ=255)

                                 gCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTcgcg                                   <  1:3106566/56‑1 (MQ=255)

                                    tGGGCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGa                              <  1:3234380/58‑1 (MQ=255)

                                       gCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGctct                                       <  1:61342/46‑1 (MQ=255)

                                       gCGTTGATTCGCAACCTGCCTGGTTTGCTGGAAGTGCTGGTGctct                                       <  1:1166960/46‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:2973011/54‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:1943834/54‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:1911868/54‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:1584857/54‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:909350/54‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:1818025/54‑1 (MQ=255)

                                         gTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:2138012/54‑1 (MQ=255)

                                          ttGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGaa                             <  1:1727896/53‑1 (MQ=255)

                                            gATTCGCAACCTGCCTGGTTTACTGGAAGTGTTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgt            >  1:930734/1‑68 (MQ=255)

                                            gATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgt            >  1:2133051/1‑68 (MQ=255)

                                            gATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgt            >  1:3343852/1‑68 (MQ=255)

                                            gATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgt            >  1:2414438/1‑68 (MQ=255)

                                            gATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgt            >  1:2942796/1‑68 (MQ=255)

                                              ttCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:1674441/68‑1 (MQ=255)

                                              ttCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:2556098/68‑1 (MQ=255)

                                              ttCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCACGGCGcgtcg          <  1:158219/68‑1 (MQ=255)

                                               tCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:445790/67‑1 (MQ=255)

                                                cGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGCGTCGTAt       >  1:1988504/1‑69 (MQ=255)

                                                cGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGCGTCGTAt       >  1:2174124/1‑69 (MQ=255)

                                                     cctgcctgGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGCGTCGTATGCCAt  <  1:1613660/69‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:198277/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:616773/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:553207/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:3289803/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:1538623/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:968240/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:103709/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:2579422/56‑1 (MQ=255)

                                                          ctgGTTTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:2683505/56‑1 (MQ=255)

                                                          ctgGTCTGCTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGcgtcg          <  1:2191008/56‑1 (MQ=39)

AGTCTGTTGTTTGCGATTATCGCCTCAATGGTGGCCTGGGCGTTGATTCGCAACCTGCCTGGTTTACTGGAAGTGCTGGTGCTCTCGCGACTGAATATGCGCCAGGGCGCGTCGTATGCCAT  >  minE/317537‑317658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A