Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
354,219 |
A→G |
24.4% |
P178P (CCA→CCG) |
cysS → |
cysteinyl‑tRNA synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 354,219 | 0 | A | G | 24.4%
| 145.2
/ 42.3
| 119 | P178P (CCA→CCG) | cysS | cysteinyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (50/39); new base G (17/12); total (68/51) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
Only 100 of 119 total aligned reads displayed.
AGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGG > minE/354158‑354279
|
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:1670794/1‑66 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:956290/1‑66 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:244028/1‑66 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATg > 1:1316307/1‑65 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:938568/1‑69 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:2240989/1‑69 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:135487/1‑69 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:203896/1‑69 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:1706227/1‑69 (MQ=255)
aGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACt > 1:1000664/1‑69 (MQ=255)
gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:61843/69‑1 (MQ=255)
gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:325632/69‑1 (MQ=255)
gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:2797215/69‑1 (MQ=255)
gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:2555750/69‑1 (MQ=255)
gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:2174658/69‑1 (MQ=255)
gCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1796752/69‑1 (MQ=255)
caggctggCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1308686/65‑1 (MQ=255)
caggcaggCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1007797/65‑1 (MQ=255)
caggcaggCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1372126/65‑1 (MQ=255)
caggcaggCGCGCGCGTTGACGTGGGCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1472008/65‑1 (MQ=255)
ggcaggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGt > 1:2166890/1‑68 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:97766/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:1317385/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:2531926/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:185581/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTc > 1:653914/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:330867/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:106732/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:635605/1‑66 (MQ=255)
aggcGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTc > 1:730615/1‑66 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAAt > 1:1025390/1‑39 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATgg > 1:2469725/1‑41 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATg > 1:2500638/1‑40 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:2777846/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:2547181/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:312134/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:36842/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:3288541/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:1836659/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:1735512/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:741292/1‑69 (MQ=255)
cgcgcgcgTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAaga > 1:747763/1‑69 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACtt > 1:2429038/1‑35 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGtt > 1:2624533/1‑39 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:2683209/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:3084166/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:549147/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1250210/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:736082/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1049578/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1550898/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:2317606/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1948838/1‑40 (MQ=255)
gACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTc > 1:1357475/1‑40 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:3171272/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:253220/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:3103028/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:2583872/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:1323459/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:1385910/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:271400/1‑69 (MQ=255)
gTGGTCGATGACAAACGTAACCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc > 1:2854343/1‑69 (MQ=255)
gTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGaa < 1:1718131/44‑1 (MQ=255)
ggTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:2382000/69‑1 (MQ=255)
ggTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:2723788/69‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1753295/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1686094/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1812161/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:811256/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:342903/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:2095796/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1673709/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:3270814/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1670195/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1084824/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:3095181/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:230083/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:2975167/60‑1 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:2176943/1‑69 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:864206/1‑69 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:1962489/1‑69 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTgg > 1:298108/1‑69 (MQ=255)
gTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:1785776/68‑1 (MQ=255)
tCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:2953829/59‑1 (MQ=255)
tCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGaa < 1:1924824/59‑1 (MQ=255)
acAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTg < 1:524019/61‑1 (MQ=255)
acAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGtctc < 1:2082216/69‑1 (MQ=255)
cAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGtctc < 1:559802/68‑1 (MQ=255)
cGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTgg > 1:1297734/1‑69 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2387406/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:83637/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2339712/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:3246438/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:1648309/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2988961/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:407226/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:347277/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:2150937/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:3422843/50‑1 (MQ=255)
aCCCGATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGc < 1:211268/50‑1 (MQ=255)
|
AGGATCTCGACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGACTTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGG > minE/354158‑354279
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
