Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
493,466 |
A→G |
22.5% |
L195L (CTT→CTC) |
gpmA ← |
phosphoglyceromutase 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 493,466 | 0 | A | G | 22.5%
| 168.5
/ 41.3
| 128 | L195L (CTT→CTC) | gpmA | phosphoglyceromutase 1 |
| Reads supporting (aligned to +/- strand): ref base A (55/44); new base G (7/22); total (62/66) |
| Fisher's exact test for biased strand distribution p-value = 3.19e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 129 total aligned reads displayed.
TCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGAT > minE/493401‑493513
|
tCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGa < 1:2730258/69‑1 (MQ=255)
tCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGa < 1:1259158/69‑1 (MQ=255)
tCATACACCACCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGa < 1:1440245/69‑1 (MQ=255)
aTAAACCAGCGGCGCGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata > 1:2220092/1‑69 (MQ=255)
aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata > 1:3396864/1‑69 (MQ=255)
aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata > 1:2225988/1‑69 (MQ=255)
aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata > 1:52256/1‑69 (MQ=255)
aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTCTTCTTCGCTCATGTTATCGAGata > 1:2918793/1‑69 (MQ=255)
aTAAACCAGCGGCACGCCGGTCGGGATATTCAGCTCAAGAATCTATTCTTCGCTCATGTTATCGAGata > 1:607464/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCa > 1:799564/1‑61 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:2815443/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:2861556/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:431609/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:2506868/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:408760/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:3349125/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:604935/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:2078115/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:2138636/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:1845784/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt < 1:1716723/69‑1 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt < 1:784128/69‑1 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:103982/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt < 1:1332250/69‑1 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:1187565/1‑69 (MQ=255)
cacCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:1126026/1‑69 (MQ=255)
ccAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc < 1:3424528/68‑1 (MQ=255)
ccAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc < 1:3122361/68‑1 (MQ=255)
ccAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTc < 1:105548/68‑1 (MQ=255)
aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat < 1:730734/57‑1 (MQ=255)
aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat > 1:1189579/1‑57 (MQ=255)
aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat > 1:2773346/1‑57 (MQ=255)
aCGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat > 1:2608027/1‑57 (MQ=255)
aCGCCAGTCGGGACATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat < 1:218321/57‑1 (MQ=255)
cGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGatat < 1:3004080/56‑1 (MQ=255)
ccAGTCGGTATATTAAGCTCAAGAATCTATTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg > 1:2874851/1‑68 (MQ=255)
ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg > 1:3388046/1‑68 (MQ=255)
ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg > 1:286435/1‑68 (MQ=255)
ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg > 1:1552145/1‑68 (MQ=255)
ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg > 1:2558449/1‑68 (MQ=255)
ccAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACg > 1:1271974/1‑68 (MQ=255)
tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag < 1:1657979/69‑1 (MQ=255)
tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag < 1:2290050/69‑1 (MQ=255)
tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag < 1:471626/69‑1 (MQ=255)
tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag < 1:1841700/69‑1 (MQ=255)
tCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGAGCACGTAAag < 1:2285721/69‑1 (MQ=255)
ttAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCg > 1:872368/1‑69 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTGATGTTATCAAGATATTTCACCAGCGCACGTa > 1:274524/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:2289981/1‑42 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:1868451/1‑42 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATAttt > 1:274128/1‑42 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGt > 1:2360653/1‑55 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa < 1:1909218/56‑1 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa < 1:1210464/56‑1 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa > 1:1998138/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa < 1:3103665/56‑1 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa > 1:13823/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa > 1:2465035/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa > 1:1781835/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa > 1:1781092/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTa > 1:1606149/1‑56 (MQ=255)
aaGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCgtg > 1:1934677/1‑69 (MQ=255)
gAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGc < 1:2809498/67‑1 (MQ=255)
gAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGc < 1:643964/67‑1 (MQ=255)
aTCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAag < 1:797373/49‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCAGGTAACGAGTTACCGTGTGCGGCgatga < 1:2326964/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:3190700/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2963255/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:1342709/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:1641599/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:1242029/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2743773/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:126108/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2614553/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2000539/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2397630/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2396325/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:478198/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:1324722/69‑1 (MQ=255)
tctcTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatga < 1:1780701/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:718011/68‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:104578/68‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2274813/68‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2120006/68‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2061143/68‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:1444079/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:3267254/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat > 1:2907499/1‑69 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:1564634/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:2355062/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:1965788/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:645421/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat < 1:1654999/69‑1 (MQ=255)
ctctTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGAgatgat < 1:5299/69‑1 (MQ=255)
cGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgat < 1:408664/58‑1 (MQ=255)
cGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgat < 1:1877513/58‑1 (MQ=255)
tCATGTTATCGAGATATTTCACCAGCGCACGTAACGAGTTACCGTGTGCGGCgatga < 1:2855308/57‑1 (MQ=255)
ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat > 1:1931395/1‑53 (MQ=255)
ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat > 1:3359533/1‑53 (MQ=255)
ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCgatgat > 1:1386858/1‑53 (MQ=255)
|
TCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGAT > minE/493401‑493513
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
