Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
498,939 |
C→G |
37.5% |
L435L (CTG→CTC) |
modF ← |
fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 498,939 | 0 | C | G | 37.5%
| 37.7
/ 48.1
| 56 | L435L (CTG→CTC) | modF | fused subunits of molybdate transporter and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (21/14); new base G (8/13); total (29/27) |
| Fisher's exact test for biased strand distribution p-value = 1.68e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 |
CAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAA > minE/498872‑498980
|
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2238754/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:3282316/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:3315671/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2792827/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2748020/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:2646899/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:247304/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:492365/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:123765/1‑69 (MQ=255)
caaacaaCAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCa > 1:1270720/1‑69 (MQ=255)
tGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGc > 1:1014085/1‑69 (MQ=255)
ttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTg < 1:410880/69‑1 (MQ=255)
cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:1292758/68‑1 (MQ=255)
cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2903530/68‑1 (MQ=255)
cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2859579/68‑1 (MQ=255)
cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2733672/68‑1 (MQ=255)
cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:2624943/68‑1 (MQ=255)
cTTAGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGt < 1:1943547/68‑1 (MQ=255)
cTAATCAGCACATCAAGAAACCGGCGGATAAGCTGTCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1998065/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:123339/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1424623/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2651521/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:3430382/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1762098/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1610454/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:1699083/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:3330898/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2224863/68‑1 (MQ=255)
cTAATCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2104729/68‑1 (MQ=255)
aaTCAGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:629693/66‑1 (MQ=255)
aTCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCa > 1:464077/1‑66 (MQ=255)
cAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCgag < 1:2606357/69‑1 (MQ=255)
aGCACATCAACAAACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTc < 1:2517250/62‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGc > 1:2147243/1‑42 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:2921784/1‑69 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:1183175/1‑69 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:1650101/69‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:47104/69‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:2508704/69‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:2502908/69‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:2434371/1‑69 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:24242/69‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc < 1:2418309/69‑1 (MQ=255)
tCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGc > 1:2073091/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:938710/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:3152721/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:3055375/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:2988793/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:2814663/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1868272/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1794305/1‑69 (MQ=255)
aacaaACCGGCGGATAAGCTGGCGATTGAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1171867/1‑69 (MQ=255)
aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:2799463/1‑69 (MQ=255)
aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1934770/1‑69 (MQ=255)
aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCaa > 1:1882453/1‑69 (MQ=255)
aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCa > 1:935521/1‑68 (MQ=255)
|
CAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAA > minE/498872‑498980
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
