Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
551,589 |
A→G |
34.1% |
N645N (AAT→AAC) |
ybiW ← |
predicted pyruvate formate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 551,589 | 0 | A | G | 34.1%
| 84.5
/ 67.3
| 117 | N645N (AAT→AAC) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base A (24/53); new base G (18/22); total (42/75) |
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
Only 100 of 117 total aligned reads displayed.
TTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTA > minE/551524‑551646
|
ttAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTa > 1:55803/1‑69 (MQ=255)
ttAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTa > 1:856210/1‑69 (MQ=255)
gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:697927/69‑1 (MQ=255)
gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:1130763/69‑1 (MQ=255)
aCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:801262/68‑1 (MQ=255)
tACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcg > 1:2664738/1‑68 (MQ=255)
aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:2879422/69‑1 (MQ=255)
aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgtc < 1:1754245/69‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATg > 1:2817336/1‑48 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1040270/1‑68 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:1447333/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2727031/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:308712/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:2650064/1‑68 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:241519/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:632148/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:468607/1‑68 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2274804/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:1380783/1‑68 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3251052/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:889548/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2756524/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:308617/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2968801/68‑1 (MQ=255)
cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:877861/68‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatt < 1:1618396/69‑2 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1811461/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1139952/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:239740/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2647858/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:3275337/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:656589/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2675630/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1667197/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1520178/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1433513/69‑1 (MQ=255)
gcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:384010/67‑1 (MQ=255)
gTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:3172423/65‑1 (MQ=255)
aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2842351/63‑1 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:2868709/1‑43 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:962830/1‑43 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:2248421/1‑43 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1358796/1‑43 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1591219/1‑43 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATgg > 1:1726306/1‑43 (MQ=255)
aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2505662/64‑1 (MQ=255)
aGTTCCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2034816/60‑1 (MQ=255)
gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:1218173/69‑1 (MQ=255)
gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:1240856/69‑1 (MQ=255)
gTTGCCGCCAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:788999/69‑1 (MQ=255)
gTTGCCGCCAATCGGACCACGACCGTAGCGCGCGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:425944/69‑1 (MQ=255)
gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:1882143/1‑68 (MQ=255)
gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:3076916/1‑68 (MQ=255)
tGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:644013/57‑1 (MQ=255)
ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1263993/57‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2627800/69‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1358155/69‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2940394/69‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1190083/69‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:3156883/69‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2682453/69‑1 (MQ=255)
gccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1154880/69‑1 (MQ=255)
ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1919200/68‑1 (MQ=255)
ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1859989/68‑1 (MQ=255)
ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1243987/68‑1 (MQ=255)
ccAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:2086929/68‑1 (MQ=255)
cAATCGGACCACGCCCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:373391/1‑67 (MQ=255)
cAATCGGACCACGCCCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:639406/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:3182747/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:820418/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2659977/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1107120/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2795191/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2751404/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1256445/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2692347/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:1972055/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2095337/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc > 1:2189440/1‑67 (MQ=255)
cAATCGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAg > 1:2354645/1‑66 (MQ=255)
cGGACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTg < 1:3175431/57‑1 (MQ=255)
ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc > 1:1891343/1‑69 (MQ=255)
ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc > 1:1276452/1‑69 (MQ=255)
ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGcc > 1:2342072/1‑69 (MQ=255)
gACCACGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:1432224/61‑1 (MQ=255)
aCGACCGTAGCGCGGGTTATGGTACTGCTTCAGTTCGTCGATATAGGTCTGATAAgc < 1:3062475/57‑1 (MQ=255)
aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag < 1:76777/69‑1 (MQ=255)
aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGAcagcag < 1:1265130/69‑1 (MQ=255)
cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:1768009/42‑1 (MQ=255)
aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCga < 1:1142452/69‑2 (MQ=255)
aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:719720/69‑1 (MQ=255)
aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:489835/69‑1 (MQ=255)
aCCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:2728597/69‑1 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:2242289/69‑1 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:780962/69‑1 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:1405549/69‑1 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:1191192/69‑1 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa < 1:905594/69‑1 (MQ=255)
ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTATGATAAGCGCGAGCCAGCAGCGTa < 1:785086/69‑1 (MQ=255)
cGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGtta < 1:2861693/38‑1 (MQ=255)
|
TTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTA > minE/551524‑551646
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
