Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
556,511 |
C→T |
42.0% |
E257E (GAG→GAA) |
moeA ← |
molybdopterin biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 556,511 | 0 | C | T | 42.0%
| 18.3
/ 39.6
| 57 | E257E (GAG→GAA) | moeA | molybdopterin biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (10/23); new base T (8/16); total (18/39) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
ATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATA > minE/556449‑556571
|
aTCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCAcc < 1:2690015/68‑1 (MQ=255)
aTCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCAcc < 1:3302994/68‑1 (MQ=255)
cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:698655/68‑1 (MQ=255)
cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:2504372/68‑1 (MQ=255)
cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:2129134/68‑1 (MQ=255)
cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:1649141/68‑1 (MQ=255)
cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:1856744/68‑1 (MQ=255)
cGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCa < 1:419889/68‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1898531/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:3017527/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:2990743/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:3185615/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:274678/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:2104373/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:3400862/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:192328/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1735727/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:731475/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1414877/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:570353/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1481796/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1461643/69‑1 (MQ=255)
cAGTTTCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:621683/69‑1 (MQ=255)
cAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGa < 1:3038713/69‑1 (MQ=255)
cAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGa < 1:1116514/69‑1 (MQ=255)
cAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGa < 1:1213267/69‑1 (MQ=255)
agtttCCAGAAGGCAATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCTTCACCCACTGa < 1:1717554/65‑1 (MQ=255)
aGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACAcc < 1:422246/67‑1 (MQ=255)
aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTg < 1:1141994/69‑1 (MQ=255)
aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTg < 1:2562687/69‑1 (MQ=255)
aTCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTg < 1:1870375/69‑1 (MQ=255)
tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:2137207/61‑1 (MQ=255)
tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:384394/61‑1 (MQ=255)
tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:20752/61‑1 (MQ=255)
tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:1967463/61‑1 (MQ=255)
tcCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCgg < 1:385313/61‑1 (MQ=255)
ccAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac < 1:2845622/67‑1 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:3206900/1‑58 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:918196/1‑58 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:275273/1‑58 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:261161/1‑58 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:3006625/1‑58 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:1801977/1‑58 (MQ=255)
cAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:1318788/1‑58 (MQ=255)
cAAGAATCGTTTTGGCGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcac > 1:134649/1‑58 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGctt > 1:1452156/1‑66 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:2465795/1‑69 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:2092400/1‑69 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:75684/1‑69 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:1748377/1‑69 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:1608289/1‑69 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:591483/1‑69 (MQ=255)
aaGAATCGTTTTGGTGTAATCCGCTTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCTTg > 1:612180/1‑69 (MQ=255)
gAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATcaccac > 1:1165985/1‑58 (MQ=255)
gAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGc > 1:2082795/1‑69 (MQ=255)
tAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATa < 1:447892/69‑1 (MQ=255)
tAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATa < 1:1850942/69‑1 (MQ=255)
|
ATCGCCAGCTTCCAGAAGGCGATCTCCCCCAGCTCTTCAAGAATCGTTTTGGTGTAATCCGCCTCACCCACTGAAACACCGCCGGAACTGATCACCACATCCGCCTGGCTGTCGGCTTCAATA > minE/556449‑556571
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
