Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
564,523 |
T→A |
25.6% |
A126A (GCA→GCT) |
ybjL ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 564,523 | 0 | T | A | 25.6%
| 60.0
/ 20.8
| 43 | A126A (GCA→GCT) | ybjL | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base T (5/27); new base A (3/8); total (8/35) |
| Fisher's exact test for biased strand distribution p-value = 4.01e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 |
TTCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAT > minE/564457‑564585
|
ttCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCt > 1:130314/1‑67 (MQ=255)
ttCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTg > 1:1553852/1‑68 (MQ=255)
cATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGATCCTGCTaa > 1:1022009/1‑69 (MQ=255)
cATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTaa > 1:1855170/1‑69 (MQ=255)
cATGCCGGAATGACCCAGTGTATCGCCAGCACCGACCAGAACCTGTGTCGACGTCATAGAGCCTGCTaa > 1:2271637/1‑69 (MQ=255)
tGCCGGAATGACTCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:1455841/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:2258016/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:2023313/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:2019629/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:2440066/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:2456118/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:2588933/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:1687253/69‑1 (MQ=255)
tGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACa < 1:1510940/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:1742520/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:1596152/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:2671810/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:1462679/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:1394700/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:432741/69‑1 (MQ=255)
aaTGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:1284598/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCTACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:2794829/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:2104079/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:2178893/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:3008212/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:1888696/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:2463886/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:3004335/69‑1 (MQ=255)
tgtATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:1472298/69‑1 (MQ=255)
tATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:2055597/69‑1 (MQ=255)
tCGCCAGCACCGGCCAGAACCGGTGTCTACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:3256655/67‑1 (MQ=255)
tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:316459/67‑1 (MQ=255)
tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:2971805/67‑1 (MQ=255)
tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:118006/67‑1 (MQ=255)
tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:1888114/67‑1 (MQ=255)
tCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCaa < 1:1327437/67‑1 (MQ=255)
gCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAatat > 1:831783/1‑68 (MQ=255)
ccAGAACCGGTGTCGACGTCATAGAGCCAGCTAACATACCgg < 1:262834/42‑1 (MQ=255)
cGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGcc < 1:486175/45‑1 (MQ=255)
aTAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCg < 1:2935943/69‑1 (MQ=255)
aTAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCg < 1:2467908/69‑1 (MQ=255)
agagCCAGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTa > 1:1991591/1‑62 (MQ=255)
agagCCAGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAt > 1:2782037/1‑69 (MQ=255)
agagCCAGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAt > 1:1895600/1‑69 (MQ=255)
|
TTCCATGCCGGAATGACGCAGTGTATCGCCAGCACCGACCAGAACCGGTGTCGACGTCATAGAGCCTGCTAACATACCGGCCGTCAGGCCAATATCCCAGCCAAACAGCTTACCTAACCCTAAGGCGAT > minE/564457‑564585
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
