Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
579,722 |
C→T |
22.3% |
Y37Y (TAC→TAT) |
clpS → |
regulatory protein for ClpA substrate specificity |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 579,722 | 0 | C | T | 22.3%
| 109.2
/ 22.9
| 80 | Y37Y (TAC→TAT) | clpS | regulatory protein for ClpA substrate specificity |
| Reads supporting (aligned to +/- strand): ref base C (37/25); new base T (6/12); total (43/37) |
| Fisher's exact test for biased strand distribution p-value = 6.24e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 105 total aligned reads displayed.
GCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGC > minE/579672‑579789
|
gcgcTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGtt < 1:3259888/69‑1 (MQ=255)
gcgcTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGtt < 1:1908401/69‑1 (MQ=255)
aaaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGa > 1:3068745/1‑69 (MQ=255)
aaaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGa > 1:181632/1‑69 (MQ=255)
aaaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGa > 1:2190711/1‑69 (MQ=255)
aaaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGa > 1:1967968/1‑69 (MQ=255)
aaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGAc > 1:3126810/1‑69 (MQ=255)
aaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGAc > 1:3432397/1‑69 (MQ=255)
aaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGAc > 1:2603292/1‑69 (MQ=255)
aaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGAc > 1:1889819/1‑69 (MQ=255)
aaaCCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGAc > 1:2021916/1‑69 (MQ=255)
aaaCCGCAATCTATGTATAAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGAc > 1:85192/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTacac > 1:1787133/1‑36 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTacac > 1:33366/1‑36 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTacac > 1:3285528/1‑36 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTacac > 1:735140/1‑36 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTacac > 1:1255136/1‑36 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTacac > 1:2293609/1‑36 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTaca > 1:2089646/1‑35 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATTGAGTTTGTTATTGACGTGTTACaaaa > 1:3280148/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt > 1:2049903/1‑55 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt > 1:3146429/1‑55 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt > 1:2051455/1‑55 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt > 1:1207400/1‑55 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTAtt > 1:500267/1‑55 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACaaaa > 1:2472953/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACaaaa > 1:290173/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACaaaa > 1:2601490/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACaaaa > 1:3240550/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACaaaa > 1:1177562/1‑69 (MQ=255)
tATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACaaa > 1:1180579/1‑68 (MQ=255)
tATGTAAAAAGTGATATTAGTCAATGATGATTacac > 1:8000/1‑36 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2662567/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2506309/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:1686237/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:269190/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2693543/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2745278/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2772207/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:3035136/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2442705/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:1649272/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:1852120/69‑1 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACTAAAAttc > 1:918627/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:2813972/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:841318/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:2817833/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:3130668/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:3183289/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:548911/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:6248/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:652471/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:3283437/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:101615/1‑69 (MQ=255)
tataAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc > 1:2320053/1‑69 (MQ=255)
ataAAGTGATATTAGTCAATGATGATTATACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttc < 1:2896146/68‑1 (MQ=255)
ttAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttct < 1:2459976/63‑1 (MQ=255)
ttAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttct < 1:3147141/63‑1 (MQ=255)
ttAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAAttcttttct < 1:1388721/63‑1 (MQ=255)
ttAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatga > 1:407401/1‑68 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:1049977/68‑1 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:3370515/68‑1 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:706028/68‑1 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:836278/68‑1 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:1474449/68‑1 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:2866011/68‑1 (MQ=255)
aGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTatgatg < 1:1214313/68‑1 (MQ=255)
aaTGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGaa > 1:2117367/1‑69 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:1996999/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:1480471/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:49105/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:1387860/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:1599118/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:1768790/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:1287206/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:819344/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:2306790/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:2577558/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:3156980/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAg < 1:2813746/63‑1 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGt > 1:1302939/1‑68 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGt > 1:1417128/1‑68 (MQ=255)
atgatTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGt > 1:480277/1‑68 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:924720/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:1225106/69‑1 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:1155130/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:1153455/69‑1 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:1092465/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:607127/69‑1 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:440475/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:407892/69‑1 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:39546/69‑1 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:3429057/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:1783904/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:206273/69‑1 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:3272500/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:2612486/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:3209266/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc > 1:2732798/1‑69 (MQ=255)
acacTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGc < 1:2789882/69‑1 (MQ=255)
|
GCGCTAAAACCGCCATCTATGTATAAAGTGATATTAGTCAATGATGATTACACTCCGATGGAGTTTGTTATTGACGTGTTACAAAAATTCTTTTCTTATGATGTAGAACGTGCAACGC > minE/579672‑579789
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
