Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
591,494 |
A→G |
23.1% |
P524P (CCA→CCG) |
ftsK → |
DNA‑binding membrane protein required for chromosome resolution and partitioning |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 591,494 | 0 | A | G | 23.1%
| 109.0
/ 27.6
| 86 | P524P (CCA→CCG) | ftsK | DNA‑binding membrane protein required for chromosome resolution and partitioning |
| Reads supporting (aligned to +/- strand): ref base A (27/39); new base G (10/10); total (37/49) |
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 |
TGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGT > minE/591437‑591538
|
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:992537/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:979117/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:93249/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3388159/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3333613/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3205927/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:3109781/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:2921323/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:2821666/1‑69 (MQ=255)
tgaacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCaa > 1:1331677/1‑69 (MQ=255)
aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1008427/1‑69 (MQ=255)
aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:878847/1‑69 (MQ=255)
aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:3160256/1‑69 (MQ=255)
aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1891916/1‑69 (MQ=255)
aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1799185/1‑69 (MQ=255)
aacgtgaacAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAAt > 1:1274909/1‑69 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1636853/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:993666/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:840089/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:581053/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1143927/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:3251482/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:3191618/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:3150189/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:2676211/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:2437615/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1437090/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1785000/1‑68 (MQ=255)
aacaacTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCg > 1:1484125/1‑68 (MQ=255)
ttGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAg < 1:1140757/69‑1 (MQ=255)
ttGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAg < 1:92276/69‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAagaaccagaac > 1:253529/1‑36 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGAAAGCAGCTTCTGtt < 1:1924750/68‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGtt < 1:3140996/68‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCg > 1:1120354/1‑38 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:1626873/1‑67 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1902401/67‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:912975/67‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:530406/67‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:1758481/1‑67 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:1763028/1‑67 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1278271/67‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:2061715/67‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:2062451/67‑1 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt > 1:2608435/1‑67 (MQ=255)
aTCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:2440580/67‑1 (MQ=255)
aaCCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTgc > 1:2599332/1‑67 (MQ=255)
aaCCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTgc > 1:2952874/1‑67 (MQ=255)
aaCCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTgc > 1:2994567/1‑67 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:2601153/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:513010/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1786686/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1229149/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:554182/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:681733/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1890828/48‑1 (MQ=255)
cGATTCCAGAACCGGTTAAAGAACCGGAACCGATCAAATCTTCGCTGa < 1:1987217/48‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:527110/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:1005421/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2762533/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:3169108/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2831100/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2834891/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:1217871/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCTAATCTTCGCTGAAAGCACATTCTGTTGCAGCAGt < 1:2132138/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGTACCTTCTGTTGCAGCAGt < 1:3373899/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGTACCTTCTGTTGCAGCAGt < 1:1707451/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:3102832/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:1143801/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:854648/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2273379/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2934683/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:675821/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:618675/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:3101848/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:541268/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:515680/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2823543/69‑1 (MQ=255)
gATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:121076/69‑1 (MQ=255)
aTTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGTACCTTCTGTTGCAGCAGt < 1:2442144/68‑1 (MQ=255)
aTTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGt < 1:2601626/68‑1 (MQ=255)
tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:3223745/45‑1 (MQ=255)
tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1681830/45‑1 (MQ=255)
tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:644275/45‑1 (MQ=255)
tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:374778/45‑1 (MQ=255)
tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:1186634/45‑1 (MQ=255)
tAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGt < 1:3294034/45‑1 (MQ=255)
accataaccGATCTAATCTTCGCTGAAAGCACATTCTGTTGCAGCAGt < 1:27510/48‑1 (MQ=255)
|
TGAACGTGAACAACTTGCGGCCTGGTATCAACCGATTCCAGAACCGGTTAAAGAACCAGAACCGATCAAATCTTCGCTGAAAGCACCTTCTGTTGCAGCAGT > minE/591437‑591538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
