Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
597,078 |
T→C |
33.7% |
L318L (TTG→CTG) |
serS → |
seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 597,078 | 0 | T | C | 33.7%
| 48.5
/ 35.7
| 68 | L318L (TTG→CTG) | serS | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Reads supporting (aligned to +/- strand): ref base T (21/24); new base C (15/8); total (36/32) |
| Fisher's exact test for biased strand distribution p-value = 2.01e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAA > minE/597011‑597143
|
cGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt > 1:2671979/1‑69 (MQ=255)
cGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt > 1:2935544/1‑69 (MQ=255)
cGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt > 1:452045/1‑69 (MQ=255)
cGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt > 1:538749/1‑69 (MQ=255)
cGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGtt > 1:245689/1‑69 (MQ=255)
cccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg < 1:3179590/69‑1 (MQ=255)
cccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg < 1:2038615/69‑1 (MQ=255)
cccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg < 1:2365301/69‑1 (MQ=255)
cccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg < 1:2490958/69‑1 (MQ=255)
cccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTggg < 1:2606129/69‑1 (MQ=255)
cccAGAAGACTCAATGGCGGCGCTGGAAGAGATGACT‑GTCATGCAGAAAAAGTCCTGCAGTTGCTggg < 1:2033566/68‑1 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTg > 1:2188414/1‑68 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:902301/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:2492564/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:3184778/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:2530660/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:2543834/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:3189026/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:3299549/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:3428343/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:1786972/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:1182704/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:1765082/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:674390/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGc > 1:1606220/1‑69 (MQ=255)
aagaCTCAATGGCGGCGCTGGAAGAGATGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTAGGCCTGc > 1:3361426/1‑69 (MQ=255)
tCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgtac > 1:381335/1‑69 (MQ=255)
tCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgtac > 1:1758087/1‑69 (MQ=255)
tCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGccgtac > 1:624058/1‑69 (MQ=255)
ggcggcGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTaa > 1:1996801/1‑69 (MQ=255)
gcggcgCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTaaa > 1:2054611/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:1485938/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:1169369/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:661577/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:1435293/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:1486179/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:586642/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:1695906/1‑69 (MQ=255)
gagaTGACTGGTCATGCAGAAAAAGTCATGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGc > 1:2213891/1‑69 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:3266827/69‑2 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:3060016/69‑2 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:1115666/69‑2 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:2726769/69‑2 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:2373031/69‑2 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:1660813/69‑2 (MQ=255)
gagaTGACCGGTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTgt < 1:2224994/69‑2 (MQ=255)
tGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGa < 1:1228971/69‑1 (MQ=255)
cggTCATGCGGAAAAAGTCCTGCAGCTGCTGGGCCTGCCGTACCGTAAAATCATGCTTTgt < 1:372160/60‑2 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:2674514/69‑1 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:997984/69‑1 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:789595/69‑1 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:713607/69‑1 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:136985/69‑1 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:2430499/69‑1 (MQ=255)
ggTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGAc < 1:2305991/69‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:945173/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:1066129/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:1806073/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:3260277/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:1728658/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:696814/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:470000/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAatca < 1:3067264/51‑1 (MQ=255)
gTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGCACCGTAAAatca < 1:1412614/51‑1 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTaaa > 1:2688980/1‑41 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:1673981/1‑69 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:528350/1‑69 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:1685174/1‑69 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:29032/1‑69 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:182658/1‑69 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:1949306/1‑69 (MQ=255)
cAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCt > 1:2163121/1‑69 (MQ=255)
gTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCaaa < 1:2590995/67‑1 (MQ=255)
|
CGTGCGCCCAGAAGACTCAATGGCGGCGCTGGAAGAGATGACTGGTCATGCAGAAAAAGTCCTGCAGTTGCTGGGCCTGCCGTACCGTAAAATCATCCTTTGCACTGGCGACATGGGCTTTGGCGCTTGCAAA > minE/597011‑597143
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
