Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 601,089 A→G 53.1% I120V (ATA→GTA) ‡ dmsC → dimethyl sulfoxide reductase, anaerobic, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE601,0890AG53.1% 15.5 / 99.0 115I120V (ATA→GTA) ‡dmsCdimethyl sulfoxide reductase, anaerobic, subunit C
Reads supporting (aligned to +/- strand):  ref base A (36/18);  new base G (27/34);  total (63/52)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 115 total aligned reads displayed.

GGCGGCATCGGCTGGTTGCTGGCAATGCTGAAAAAGCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATAC  >  minE/601023‑601156
                                                                  |                                                                   
ggcggcCTCGGCTGGTTGCTGGCAATGCTGAAAAAGCTGTCACCGGCATTGCGTACGCTGTGGCTGATa                                                                   >  1:440783/1‑69 (MQ=255)
ggcggcATCGGCTGGTTGCTGGCAATGCTGAAAAAGCTGTCACCGGCATTGCGTACGCTGTGGCTGATa                                                                   >  1:1217183/1‑69 (MQ=255)
ggcggcATCGGCTGGTTGCTGGCAATGCTGAAAAAGCTGTCACCGGCATTGCGTACGCTGTGGCTGATa                                                                   >  1:833371/1‑69 (MQ=255)
                tGCTGGCAATGCTGAAAAAGCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTg                                                   >  1:133882/1‑69 (MQ=255)
                                  aGCTGTCACCGGCATTGCGTACGCTGTGGCTGATa                                                                   >  1:2879993/1‑35 (MQ=255)
                                  aGCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTgg                                  >  1:948570/1‑68 (MQ=255)
                                   gCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgat                                <  1:21124/69‑1 (MQ=255)
                                      gTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatgat                             <  1:2258283/69‑1 (MQ=255)
                                         aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                          >  1:2601647/1‑69 (MQ=255)
                                         aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                          >  1:2336322/1‑69 (MQ=255)
                                         aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                          >  1:2960521/1‑69 (MQ=255)
                                         aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                          >  1:2125136/1‑69 (MQ=255)
                                         aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                          >  1:1961908/1‑69 (MQ=255)
                                         aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                          >  1:33733/1‑69 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTTgatg                               <  1:1415835/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:2533688/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:1460145/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:3248437/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:2404574/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:1668440/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:396593/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:2257266/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:1846060/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:748152/60‑1 (MQ=255)
                                             gCATTGCGTACGCTGTGGCTGATAGTGACGACGGTTCTTGGCGTCATCTTTGTCTGgatg                               <  1:2889558/60‑1 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:1730095/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:2104741/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:1923778/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:1828013/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:3364863/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:773723/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:2294691/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:2419895/1‑68 (MQ=255)
                                                  gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                  >  1:145662/1‑68 (MQ=255)
                                                         cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc          >  1:1458106/1‑69 (MQ=255)
                                                         cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc          >  1:2965739/1‑69 (MQ=255)
                                                         cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc          >  1:1458353/1‑69 (MQ=255)
                                                         cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc          >  1:1505765/1‑69 (MQ=255)
                                                         cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc          >  1:1391555/1‑69 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCTTGTGTATAACAGCa         <  1:645763/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACTGCa         <  1:9245/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:625892/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:1072355/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2679049/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:475721/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:268503/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2788760/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:588121/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2794158/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:3371814/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:3350981/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:3295061/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2926425/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:627415/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:3261523/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2976794/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:3209287/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:1751828/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2114292/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2079640/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2180958/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2218039/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:1384830/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:13493/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:1230327/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:1917887/67‑1 (MQ=255)
                                                          tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGAGTGTGTATAACAGCa         <  1:1627858/67‑1 (MQ=255)
                                                          tttgGCTGGTCGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:3162993/67‑1 (MQ=255)
                                                           ttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa         <  1:2292559/67‑1 (MQ=255)
                                                           ttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGCTGCGTGTGTATAACAGCa         <  1:1945619/67‑1 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGGGTGTGTATAACAGCAtc       >  1:2337063/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGtata               >  1:1600481/1‑61 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1693096/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1441583/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1671199/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:398408/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1006251/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1434636/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1222381/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1160505/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2672347/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2142309/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2561646/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2730773/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:279901/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2869017/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:1826153/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2963665/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:212007/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:2117401/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:3015364/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc       >  1:3260816/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt        >  1:2778861/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt        >  1:2345412/1‑68 (MQ=255)
                                                            tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt        >  1:3344821/1‑68 (MQ=255)
                                                              gCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatga                              >  1:2140380/1‑44 (MQ=255)
                                                              gCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatga                              >  1:700746/1‑44 (MQ=255)
                                                              gCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatga                              >  1:1260402/1‑44 (MQ=255)
                                                              gCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatga                              >  1:762225/1‑44 (MQ=255)
                                                                 gATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATAc  <  1:1711712/69‑1 (MQ=255)
                                                                  |                                                                   
GGCGGCATCGGCTGGTTGCTGGCAATGCTGAAAAAGCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATAC  >  minE/601023‑601156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A