Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 601,113 A→G 48.7% I128V (ATC→GTC)  dmsC → dimethyl sulfoxide reductase, anaerobic, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE601,1130AG48.7% ‑0.4 / 180.3 189I128V (ATC→GTC) dmsCdimethyl sulfoxide reductase, anaerobic, subunit C
Reads supporting (aligned to +/- strand):  ref base A (40/57);  new base G (27/65);  total (67/122)
Fisher's exact test for biased strand distribution p-value = 9.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 199 total aligned reads displayed.

AGCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCATCTGG  >  minE/601057‑601181
                                                        |                                                                    
    gTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatgat                                                      <  1:2258283/69‑1 (MQ=255)
       aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                                                   >  1:1961908/1‑69 (MQ=255)
       aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                                                   >  1:2336322/1‑69 (MQ=255)
       aCCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGt                                                   >  1:1449601/1‑69 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTTgatg                                                        <  1:1415835/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:2533688/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:1846060/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:1622599/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:1460145/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:1318309/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:3248437/60‑1 (MQ=255)
           gCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatg                                                        <  1:396593/60‑1 (MQ=255)
                gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGt                                                               >  1:2883073/1‑48 (MQ=255)
                gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                                           >  1:773723/1‑68 (MQ=255)
                gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                                           >  1:1923778/1‑68 (MQ=255)
                gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                                           >  1:2419895/1‑68 (MQ=255)
                gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                                           >  1:3364863/1‑68 (MQ=255)
                gCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCgtgtgt                                           >  1:2104741/1‑68 (MQ=255)
                       cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc                                   >  1:1391555/1‑69 (MQ=255)
                       cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc                                   >  1:1458106/1‑69 (MQ=255)
                       cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc                                   >  1:2121953/1‑69 (MQ=255)
                       cTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGc                                   >  1:1505765/1‑69 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:3209287/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:627415/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2079640/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2114292/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:475721/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2679049/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:3371814/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2218039/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:588121/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:1751828/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2976794/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:1230327/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:13493/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2926425/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:3261523/67‑1 (MQ=255)
                        tttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:727805/67‑1 (MQ=255)
                         ttgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCa                                  <  1:2292559/67‑1 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:2117401/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:2869017/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:1671199/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:1693096/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:279901/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:2963665/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:1826153/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:1160505/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:1006251/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:2561646/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtc                                >  1:3015364/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt                                 >  1:2778861/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt                                 >  1:3344821/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt                                 >  1:3138929/1‑68 (MQ=255)
                          tgGCTGGTGGTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAt                                 >  1:2345412/1‑68 (MQ=255)
                            gCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatga                                                       >  1:700746/1‑44 (MQ=255)
                            gCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgatga                                                       >  1:2140380/1‑44 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATTGTGCgtgtgt                                           <  1:2732921/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:2707645/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1054515/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1060781/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1187329/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:828179/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1383550/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1441183/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:720799/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1636949/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:406775/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1734093/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:2032573/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:227540/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:2152864/49‑1 (MQ=255)
                                  ggTGACGATGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:2687086/49‑1 (MQ=255)
                                  ggTGACGATGGTTATTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1267722/49‑1 (MQ=255)
                                  aGTGACGATGGTTCTTGGCGTCATCTTTGTCTGgat                                                         <  1:957619/36‑1 (MQ=255)
                                   gTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGtt                       <  1:3082275/69‑1 (MQ=255)
                                   gTGACGAAGGTTCTTGGCGTAGTCTTTGTCTGGATGATGGTGCgtgtgt                                           <  1:1888523/49‑1 (MQ=255)
                                     gACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTcc                     >  1:3172154/1‑69 (MQ=255)
                                      aCGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCg                    <  1:1101188/69‑1 (MQ=255)
                                      aCGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCg                    <  1:1480925/69‑1 (MQ=255)
                                               cTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtt                                >  1:2848246/1‑48 (MQ=255)
                                               cTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtt                                >  1:3378276/1‑48 (MQ=255)
                                               cTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtt                                >  1:3384771/1‑48 (MQ=255)
                                               cTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtt                                >  1:1410175/1‑48 (MQ=255)
                                               cTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCAtt                                >  1:3334029/1‑48 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAg         <  1:939602/66‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAg         <  1:750862/66‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAg         <  1:2911722/66‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:2941558/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:899577/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:1219780/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:737929/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:1729187/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:2241567/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:2385967/68‑1 (MQ=255)
                                                    cGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCa       <  1:2727248/68‑1 (MQ=255)
                                                        aTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGa                              >  1:1563897/1‑41 (MQ=255)
                                                        aTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCg                    >  1:2793962/1‑51 (MQ=255)
                                                        aTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGCCCTGGTACAGCATCTg   >  1:480906/1‑68 (MQ=255)
                                                        aTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCATCTg   <  1:2325123/68‑1 (MQ=255)
                                                        aTCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCATCTg   <  1:2609519/68‑1 (MQ=255)
                                                        |                                                                    
AGCTGTCACCGGCATTGCGTACGCTGTGGCTGATAGTGACGATGGTTCTTGGCGTCATCTTTGTCTGGATGATGGTGCGTGTGTATAACAGCATTGATACCGTTCCGACCTGGTACAGCATCTGG  >  minE/601057‑601181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A