Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
632,854 |
T→C |
41.0% |
F169F (TTT→TTC) |
mukE → |
protein involved in chromosome partitioning |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 632,854 | 0 | T | C | 41.0%
| 26.4
/ 56.2
| 78 | F169F (TTT→TTC) | mukE | protein involved in chromosome partitioning |
| Reads supporting (aligned to +/- strand): ref base T (21/25); new base C (5/27); total (26/52) |
| Fisher's exact test for biased strand distribution p-value = 7.16e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCAC > minE/632814‑632921
|
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:1011298/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:803705/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:2546331/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:2500510/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:2432950/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:1005437/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:3338399/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:316993/1‑67 (MQ=255)
tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:134969/1‑67 (MQ=255)
gtgtGGTTTATGGGCGACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1775584/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1857781/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:2949737/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:166842/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1312945/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:776728/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:2791634/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1231713/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:690106/1‑69 (MQ=255)
gtgtGGTTTATGGGCCACGACAGCAGCAAGTACCGCATTACCGAATCGGTCTTc > 1:935089/1‑54 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:3259947/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:20679/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:1882731/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:3128719/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:656576/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:662130/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:1435583/1‑69 (MQ=255)
gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtg < 1:1707788/68‑1 (MQ=255)
tgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg < 1:3436329/69‑1 (MQ=255)
tgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg < 1:267257/69‑1 (MQ=255)
tgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg < 1:1627230/69‑1 (MQ=255)
ttATGGTCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2606427/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2378738/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:1083539/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2546463/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:951617/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:3365140/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2043800/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:1529630/40‑1 (MQ=255)
ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:522343/40‑1 (MQ=255)
tATGGGCCACGTCAGCAGCAAGTTCCGCATTACCGAATCg < 1:2253076/40‑1 (MQ=38)
tATGGGCCACGACATCAGCAAGTTCCGCATTACCGAATCg < 1:1309213/40‑1 (MQ=38)
tATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:414131/39‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:431534/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:386965/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2726229/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:347858/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2986947/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:3045458/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:411529/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:310846/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:3185135/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:3231580/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:71980/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:536927/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1108918/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1254608/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1337923/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1365456/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1439458/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1589822/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1765329/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1835459/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1856295/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2213013/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2281150/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2356098/40‑1 (MQ=255)
tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2696813/40‑1 (MQ=255)
gcaAGTTTCGCATTACCGAATCTGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:1240389/69‑1 (MQ=255)
gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:2337339/69‑1 (MQ=255)
gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:2541418/69‑1 (MQ=255)
gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:26062/69‑1 (MQ=255)
gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:1042119/69‑1 (MQ=255)
aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2453463/69‑1 (MQ=255)
aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2961423/69‑1 (MQ=255)
aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2921630/69‑1 (MQ=255)
aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2752101/69‑1 (MQ=255)
aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:1351640/69‑1 (MQ=255)
aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:1070717/69‑1 (MQ=255)
tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:972308/69‑1 (MQ=255)
tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:622093/69‑1 (MQ=255)
tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:48717/69‑1 (MQ=255)
tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:431479/69‑1 (MQ=255)
tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:2577287/69‑1 (MQ=255)
tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:2063557/69‑1 (MQ=255)
ttCGCGTTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:2673677/1‑69 (MQ=255)
ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:598340/1‑69 (MQ=255)
ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:3323207/1‑69 (MQ=255)
ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:706097/1‑69 (MQ=255)
ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:25701/1‑69 (MQ=255)
ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:2335898/1‑69 (MQ=255)
ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:2129955/1‑69 (MQ=255)
|
TAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCAC > minE/632814‑632921
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
