Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
720,741 |
A→G |
42.5% |
V961A (GTC→GCC) |
rne ← |
fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 720,741 | 0 | A | G | 42.5%
| 18.2
/ 50.9
| 69 | V961A (GTC→GCC) | rne | fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome |
| Reads supporting (aligned to +/- strand): ref base A (7/32); new base G (17/12); total (24/45) |
| Fisher's exact test for biased strand distribution p-value = 7.80e-04 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01 |
CGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACT > minE/720676‑720806
|
cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:202668/68‑1 (MQ=255)
cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:816411/68‑1 (MQ=255)
cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:1874826/68‑1 (MQ=255)
cGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACa < 1:2382218/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCTACAACTTCTTCAat < 1:1539985/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGCCAACTTCTTCAat < 1:162527/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:100355/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:508703/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2207445/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2977031/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:1839677/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2272585/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2296945/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2315060/68‑1 (MQ=255)
gcgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:79463/68‑1 (MQ=255)
cgGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:1369979/67‑1 (MQ=255)
aacTTCAGGTTCAGCAACTTCAACCTCAGCAGTTTCGACATCTTCTTCAat < 1:1537038/51‑1 (MQ=255)
cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAActtc > 1:3160881/1‑40 (MQ=255)
cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCgg > 1:908073/1‑67 (MQ=255)
cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGt > 1:737882/1‑68 (MQ=255)
cAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGt > 1:1193390/1‑68 (MQ=255)
ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAat < 1:2052594/44‑1 (MQ=255)
ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:621707/68‑1 (MQ=255)
ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:1351700/68‑1 (MQ=255)
ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:3083223/68‑1 (MQ=255)
ggTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:3346841/68‑1 (MQ=255)
gTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTc < 1:2745595/67‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCTCCg < 1:2162500/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:3239556/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:207003/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1959192/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:566674/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:2252695/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1791205/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1767009/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:716435/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:280935/69‑1 (MQ=255)
cAACCACAACCTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:2800564/69‑1 (MQ=255)
aaCTACAACCTCAGCTGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:1964885/69‑1 (MQ=255)
aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:739118/69‑1 (MQ=255)
aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:2394705/69‑1 (MQ=255)
aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCgg < 1:2756832/69‑1 (MQ=255)
aaCTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCAACgg < 1:942005/69‑1 (MQ=255)
aCAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1204328/1‑69 (MQ=255)
aCAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2012560/1‑69 (MQ=255)
cTCAGCAGTTTCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCg < 1:1982305/59‑1 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCACCCACCGGGTCt > 1:2215482/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:400494/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1118124/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:896367/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1205229/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1229419/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1430470/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1476721/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1513170/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1905131/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:1968394/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:39724/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:3119507/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2220511/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2814148/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2337941/1‑54 (MQ=255)
tCGGCAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCt > 1:2791673/1‑54 (MQ=255)
tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:2639344/66‑1 (MQ=255)
tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:563586/66‑1 (MQ=255)
tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:1589694/66‑1 (MQ=255)
tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:2014107/66‑1 (MQ=255)
tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCa < 1:2631808/66‑1 (MQ=255)
tCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACt > 1:392383/1‑69 (MQ=255)
|
CGACTACTGGCGCGGCAGGTTGAGCAACAACTTCAGGTTCAGCAACTACAACCTCAGCAGTTTCGACAACTTCTTCAATATCTGCCGTCTCTTCCTGCGGTTCAACCACCGGTTCTGCTTGTTCTGCAACT > minE/720676‑720806
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
