Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
931,721 |
A→G |
31.5% |
N165N (AAT→AAC) |
yddG ← |
predicted methyl viologen efflux pump |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 931,721 | 0 | A | G | 31.5%
| 63.9
/ 37.2
| 76 | N165N (AAT→AAC) | yddG | predicted methyl viologen efflux pump |
| Reads supporting (aligned to +/- strand): ref base A (24/28); new base G (15/9); total (39/37) |
| Fisher's exact test for biased strand distribution p-value = 2.22e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 |
GTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAC > minE/931662‑931787
|
gTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCcg < 1:2711859/69‑1 (MQ=255)
aGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtatt < 1:2853104/69‑1 (MQ=255)
aaaCCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGtattta < 1:1834078/68‑1 (MQ=255)
cctcaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2100134/66‑1 (MQ=255)
ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:397379/69‑1 (MQ=255)
ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2205873/69‑1 (MQ=255)
ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:1815197/69‑1 (MQ=255)
ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2810786/69‑1 (MQ=255)
ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2806860/69‑1 (MQ=255)
ccACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:2619338/69‑1 (MQ=255)
cacaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGt < 1:534170/68‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:845488/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2091070/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2032990/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:198512/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2559399/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2636689/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:3076420/69‑1 (MQ=255)
acaGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:1326098/69‑1 (MQ=255)
acaGACTAGCTCCCGTTAGCAGGAGAAAAACGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTa < 1:2158599/69‑1 (MQ=255)
ttGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGc < 1:376657/69‑1 (MQ=255)
ttGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGc < 1:2117419/69‑1 (MQ=255)
gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCt < 1:2401520/69‑1 (MQ=255)
gTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCt < 1:833516/69‑1 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAg > 1:3422534/1‑65 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:2458729/1‑69 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:3136359/1‑69 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:3287022/1‑69 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:106817/1‑69 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:1644469/1‑69 (MQ=255)
cAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:1762503/1‑69 (MQ=255)
cAAAAACGGTAATTCCATTAAATCAGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATaa > 1:1667716/1‑69 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTATGCTGCCCAGATa > 1:2793897/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1895249/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:470702/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:3424525/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:614558/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:3146589/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:8306/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1480235/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2845365/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1647497/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2199774/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1821838/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2560653/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:1779443/1‑67 (MQ=255)
aaaaaCGGTAATTCCGTTAAATCCACGTGCGTATTTATTCGTTACTGTGCAGTAGGCTGCCCAGATa > 1:2409733/1‑67 (MQ=255)
aaaCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg < 1:2654154/69‑1 (MQ=255)
aaaCGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg < 1:2502361/69‑1 (MQ=255)
aaCTGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACg < 1:1778174/68‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:325339/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:458434/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1207131/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1209938/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:2171030/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1293095/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:33881/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1580137/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:687422/69‑1 (MQ=255)
ggTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCAcc < 1:1485809/69‑1 (MQ=255)
tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGaa > 1:3377981/1‑69 (MQ=255)
tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGaa > 1:2689989/1‑69 (MQ=255)
tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGaa > 1:1421991/1‑69 (MQ=255)
tCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGa > 1:524349/1‑68 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1070237/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1120555/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:661163/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:2102905/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:10550/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1392092/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3235810/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3193263/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3120090/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:3109886/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:1709393/1‑69 (MQ=255)
ccATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAc > 1:227830/1‑69 (MQ=255)
|
GTAAGAAAATAGTAAACCCACAGACTTGCTCCCGTTAGCAGGACAAAAACGGTAATTCCATTAAATCCGCGTGCGTATTTATTCGTTACTGTGCAATAGGCTGCCCAGATAAACGCACCAATGAAC > minE/931662‑931787
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
