Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,013,728 |
A→G |
37.5% |
I184I (ATT→ATC) |
fumC ← |
fumarate hydratase (fumarase C), aerobic Class II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,013,728 | 0 | A | G | 37.5%
| 57.0
/ 67.6
| 104 | I184I (ATT→ATC) | fumC | fumarate hydratase (fumarase C), aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base A (27/38); new base G (11/28); total (38/66) |
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 109 total aligned reads displayed.
GCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGG > minE/1013663‑1013796
|
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:1758764/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:3173092/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:917133/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:1995670/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:751396/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:1572542/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:3354325/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:44452/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt > 1:2272968/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAt > 1:1658082/1‑68 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGAGTGGCGTCCTGCAAGTGGGTACGACCGAt > 1:1817987/1‑68 (MQ=255)
aCCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTg > 1:1715599/1‑69 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:1462262/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:883504/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:2068589/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:3194486/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:2744252/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:1077167/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg < 1:2922034/69‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg < 1:2507121/68‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg < 1:3033150/68‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg < 1:1757534/68‑1 (MQ=255)
ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg < 1:981060/68‑1 (MQ=255)
cAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGa > 1:1275990/1‑69 (MQ=255)
gTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAgt < 1:189384/69‑1 (MQ=255)
gTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAgt < 1:906722/69‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:2930459/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:2365110/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:52678/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:1071502/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:2029662/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:863631/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:460810/59‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc < 1:3311301/69‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc > 1:904468/1‑69 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc < 1:2854213/69‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc < 1:2567392/69‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc < 1:151278/69‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc > 1:2124086/1‑69 (MQ=255)
gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc < 1:645921/69‑1 (MQ=255)
gCATCCTGCAAGTGAGTACGA‑CAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCt > 1:2640588/1‑69 (MQ=255)
gCATCCTGCAAGTGAGCACGCCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc > 1:1907453/1‑69 (MQ=255)
gCATCCTGCAAGTGAGCACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc > 1:433478/1‑69 (MQ=255)
gCATCCTGCAAGTGAGCACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc > 1:2598783/1‑69 (MQ=255)
gCAACCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc < 1:2927833/59‑1 (MQ=255)
ccTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCtgt > 1:498804/1‑68 (MQ=255)
cTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCtgt > 1:3047722/1‑67 (MQ=255)
cTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCtgt > 1:724040/1‑67 (MQ=255)
aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg < 1:2514125/69‑1 (MQ=255)
aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg < 1:142525/69‑1 (MQ=255)
aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg < 1:3367781/69‑1 (MQ=255)
aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg < 1:895411/69‑1 (MQ=255)
aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg < 1:1616141/69‑1 (MQ=255)
aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg < 1:1634908/69‑1 (MQ=255)
gAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCa > 1:725928/1‑47 (MQ=255)
gAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCa > 1:1131390/1‑47 (MQ=255)
gAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCa > 1:2309467/1‑47 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:3273517/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:685600/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:712477/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:102915/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:1913001/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:1824313/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:1584478/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:2374297/68‑1 (MQ=255)
ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:180309/68‑1 (MQ=255)
ggTAAGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:2192778/68‑1 (MQ=255)
gTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:1332287/68‑1 (MQ=255)
gTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt < 1:34563/68‑1 (MQ=255)
tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa > 1:1575443/1‑69 (MQ=255)
tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa > 1:877559/1‑69 (MQ=255)
tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa > 1:2525057/1‑69 (MQ=255)
tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa > 1:862417/1‑69 (MQ=255)
tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTAAGTGTCTGTGTCAGGGTTTTaa > 1:2915390/1‑69 (MQ=255)
cGACCGATTTTGACGATATCTGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:361852/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTGAGCGTCt < 1:3093303/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:1672772/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:1688926/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:789242/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:926929/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:2903447/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:1933544/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:2266118/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:2108796/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:2855500/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:362728/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:278332/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:2329545/52‑1 (MQ=255)
cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt < 1:250776/52‑1 (MQ=255)
cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg > 1:1002638/1‑68 (MQ=255)
cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg > 1:1505039/1‑68 (MQ=255)
cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg > 1:2759052/1‑68 (MQ=255)
cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg > 1:257066/1‑68 (MQ=255)
cGACCAATTTTGACGATATCGGCAAAACCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg > 1:139029/1‑68 (MQ=255)
aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCt < 1:375053/68‑1 (MQ=255)
aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg < 1:1603658/69‑1 (MQ=255)
aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg < 1:362549/69‑1 (MQ=255)
aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg < 1:3038794/69‑1 (MQ=255)
aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg < 1:1188023/69‑1 (MQ=255)
aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg < 1:2272089/69‑1 (MQ=255)
|
GCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGG > minE/1013663‑1013796
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
