Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,036,647 |
G→A |
35.7% |
V680I (GTT→ATT) |
rsxC → |
fused predicted 4Fe‑4S ferredoxin‑type protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,036,647 | 0 | G | A | 35.7%
| 60.5
/ 37.1
| 67 | V680I (GTT→ATT) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (27/16); new base A (7/17); total (34/33) |
| Fisher's exact test for biased strand distribution p-value = 1.12e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 |
CTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAA > minE/1036582‑1036715
|
cTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTg < 1:2441299/69‑1 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:2800490/1‑68 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:1135643/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:2550010/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:2207789/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:343848/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:2133436/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:343971/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:47651/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:1571583/1‑69 (MQ=255)
gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:153770/1‑69 (MQ=255)
aGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1557112/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1640959/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:853290/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:788752/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:216916/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:2573398/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:2109993/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1978570/69‑1 (MQ=255)
aGCCAAAGCGCGCAAACGGGAACAGCAACCGGATAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1414262/69‑1 (MQ=255)
cgcgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg > 1:304309/1‑69 (MQ=255)
ggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2869454/69‑1 (MQ=255)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2712753/2‑67 (MQ=11)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2802934/2‑67 (MQ=11)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2280364/2‑67 (MQ=11)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2375354/2‑67 (MQ=11)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2943849/2‑67 (MQ=11)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2998915/2‑67 (MQ=11)
aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:762483/2‑67 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2975563/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:3291422/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:857537/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:427945/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:955797/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2800153/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:1227361/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2421909/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2296654/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2286288/69‑4 (MQ=11)
ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2202809/69‑4 (MQ=11)
tGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:752547/63‑4 (MQ=11)
cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa > 1:2889386/1‑68 (MQ=255)
cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa > 1:748598/1‑68 (MQ=255)
cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa > 1:3334150/1‑68 (MQ=255)
cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGAGGCTATTGCACGCGCCAAAGCa > 1:308637/1‑68 (MQ=255)
ggAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2009271/48‑1 (MQ=255)
gAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2862165/47‑1 (MQ=255)
ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:2735994/69‑1 (MQ=255)
ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:604434/69‑1 (MQ=255)
ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:378281/69‑1 (MQ=255)
ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:2541943/69‑1 (MQ=255)
ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:2927672/69‑1 (MQ=255)
aagaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg < 1:3222271/69‑1 (MQ=255)
aagaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg < 1:1840138/69‑1 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCTGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1491741/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2267947/39‑1 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgcg > 1:3272631/1‑50 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1046894/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1220818/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1364305/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:769768/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:231427/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:693893/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:2311160/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1980714/1‑68 (MQ=255)
agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:380630/1‑68 (MQ=255)
agaaCAGGTTGATACGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:3113409/1‑68 (MQ=255)
gTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa < 1:3003422/69‑1 (MQ=255)
|
CTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAA > minE/1036582‑1036715
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
