Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,134,888 |
T→C |
40.6% |
V276V (GTT→GTC) |
pfkB → |
6‑phosphofructokinase II |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,134,888 | 0 | T | C | 40.6%
| 35.1
/ 64.6
| 91 | V276V (GTT→GTC) | pfkB | 6‑phosphofructokinase II |
| Reads supporting (aligned to +/- strand): ref base T (14/40); new base C (22/15); total (36/55) |
| Fisher's exact test for biased strand distribution p-value = 2.09e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-02 |
GCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAT > minE/1134830‑1134951
|
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg < 1:3374952/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg < 1:1190402/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg < 1:1676882/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg < 1:1732995/69‑1 (MQ=255)
gCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg < 1:2059465/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:965343/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:800241/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:864940/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:1664327/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:1503592/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:1932710/69‑1 (MQ=255)
cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:3205669/69‑1 (MQ=255)
cGGCGCTATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:673467/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATTCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:946416/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:57079/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:2141953/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:2537665/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:2980194/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:3157269/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:2116402/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:1228901/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:1517651/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:1119784/69‑1 (MQ=255)
cGGCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:1424284/69‑1 (MQ=255)
cGGCGCGATGACACCTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:1474269/69‑1 (MQ=255)
cGGCGCGATGAAACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:1965446/69‑1 (MQ=255)
ggCGCGATGACACTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGc < 1:415268/68‑1 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGagcagc > 1:3051312/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2506630/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2278955/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2280585/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2435953/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:494193/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2746254/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:457460/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1276333/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:277105/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2128571/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:3356277/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1781491/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1791778/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1469823/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1441551/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:3427802/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1207678/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:3178560/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:1285642/1‑69 (MQ=255)
acTTAAACTGGCAGAAAATGCCTCTCTTGAAAAGATGGTCCGt > 1:3168690/1‑43 (MQ=37)
acTTAAACTGGCAGAAAATGCCTCTCTT‑AAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGc > 1:2452090/1‑68 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGGTGGTTCGTTTTGGCGTAGCTGCgggg > 1:718935/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:3088137/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:120885/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:2983361/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:531145/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:491337/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:2607699/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:2606490/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:2533518/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:233560/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:2226233/1‑58 (MQ=255)
gAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgggg > 1:13223/1‑58 (MQ=255)
aaCTGGCAGTAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt < 1:76117/46‑1 (MQ=255)
aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAggg > 1:148575/1‑69 (MQ=255)
aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAggg > 1:1629943/1‑69 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCtg < 1:309365/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:516652/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:534394/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:1088780/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:652642/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:1546377/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:3259856/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:2087265/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:2431450/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:3171414/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:3016281/66‑1 (MQ=255)
gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGCAACACGTCtg < 1:2469919/66‑1 (MQ=255)
aaGTGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:3378984/65‑1 (MQ=255)
gaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtg < 1:15178/61‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:2476560/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:2472731/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:45504/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:405485/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:1925629/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:1638400/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:1574311/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:3266520/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:1417829/69‑1 (MQ=255)
aTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:1011620/69‑1 (MQ=255)
tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:2688317/68‑1 (MQ=255)
tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:394494/68‑1 (MQ=255)
tGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAt < 1:2062571/68‑1 (MQ=255)
|
GCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCAT > minE/1134830‑1134951
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
