Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,153,806 |
C→T |
70.1% |
S270N (AGT→AAT) |
astE ← |
succinylglutamate desuccinylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,153,806 | 0 | C | T | 70.1%
| 42.8
/ 20.9
| 50 | S270N (AGT→AAT) | astE | succinylglutamate desuccinylase |
| Reads supporting (aligned to +/- strand): ref base C (2/13); new base T (27/8); total (29/21) |
| Fisher's exact test for biased strand distribution p-value = 7.27e-05 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAT > minE/1153744‑1153863
|
gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:644422/69‑1 (MQ=255)
gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:2591555/69‑1 (MQ=255)
gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:1431788/69‑1 (MQ=255)
gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCa < 1:3251575/69‑1 (MQ=255)
gTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGACATAAAATTCAGCGTGTCACTTGCCa < 1:2635380/69‑1 (MQ=255)
gcgcCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAg < 1:43965/68‑1 (MQ=255)
ccTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACggcgagtggcg > 1:1293532/1‑68 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCTTTTGCCATATGCATTTc > 1:1370526/1‑45 (MQ=38)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCat > 1:3296033/1‑36 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2269079/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1009051/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2449735/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2624268/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2832692/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2874219/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2943769/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:325557/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:3351662/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:3378165/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:483061/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:604009/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:892976/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:225950/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2258312/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2249430/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:2113416/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1898986/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1000109/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1877620/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1817186/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1714872/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:1364187/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcga > 1:137917/1‑57 (MQ=255)
ctcAAACGGCATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACggcg > 1:1491413/1‑56 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCTAGTGGCGAGTAATTTGCGAAac < 1:143110/69‑1 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:941126/69‑1 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:80140/69‑1 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:489173/69‑1 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:2827045/69‑1 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:2755554/69‑1 (MQ=255)
cATAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:2299461/69‑1 (MQ=255)
aTAAAATTCAGCGTGTCATTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAac < 1:1577451/68‑1 (MQ=255)
aTAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAacc > 1:1124219/1‑69 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:2958784/69‑1 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:3379070/69‑1 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:411532/69‑1 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:1267461/69‑1 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:1216820/69‑1 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:2903802/69‑1 (MQ=255)
tCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAt < 1:1068130/69‑1 (MQ=255)
|
GTTCCTCTCCGTCCTGCGCCAGCAATGTTCCTTTCTCAAACGGCATAAAATTCAGCGTGTCACTTGCCATATGCATTTCGAAGGACGGCGAGTGGCGAGTAATTTGCGAAACCACCCGAT > minE/1153744‑1153863
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
