Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,179,177 |
A→G |
74.7% |
P209P (CCA→CCG) |
ansA → |
cytoplasmic L‑asparaginase I |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,179,177 | 0 | A | G | 74.7%
| 68.7
/ 19.2
| 59 | P209P (CCA→CCG) | ansA | cytoplasmic L‑asparaginase I |
| Reads supporting (aligned to +/- strand): ref base A (10/5); new base G (26/18); total (36/23) |
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 |
TTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTGCGCAATTTT > minE/1179110‑1179237
|
ttCGTCGTTTGAATACGCCACCCGCCCCGCTCGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:141285/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3331015/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:207845/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2945645/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3085129/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2091867/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3095967/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2280430/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:370440/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:2750736/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:597804/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:610276/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:694236/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:1448115/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:781801/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:3422776/1‑69 (MQ=255)
ttCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCcac > 1:1217556/1‑69 (MQ=255)
cgtTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAAcc < 1:3219146/68‑1 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:2749567/1‑69 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:581399/1‑69 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:771047/1‑69 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:3110864/1‑69 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:2176797/1‑69 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:426595/1‑69 (MQ=255)
tGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTg > 1:3398590/1‑69 (MQ=255)
tACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGt < 1:2806031/69‑1 (MQ=255)
aCCCGTCCCGCCCGGTGAAGGGGCATTGATCGTTCATCCAATCACCCCGCAACCAATTg > 1:1928423/1‑59 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGa > 1:3196625/1‑68 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2392907/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2708675/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2697765/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2691434/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:1332834/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2563275/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:1507378/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2069469/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2758716/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:3146297/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:1852521/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:210540/1‑69 (MQ=255)
aCCCGTCCCGCACGGTGAAGGGGCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCGTAGTGAc > 1:2910662/1‑69 (MQ=255)
cgccccgcACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGa > 1:353751/1‑68 (MQ=255)
cgccccgcACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGa > 1:3105302/1‑68 (MQ=255)
ggTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAggg < 1:3333376/69‑1 (MQ=255)
ggTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAggg < 1:1889677/69‑1 (MQ=255)
ggTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAggg < 1:1177715/69‑1 (MQ=255)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCaa > 1:2062139/1‑37 (MQ=21)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGc > 1:1625688/1‑42 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1006061/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:2352389/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:422942/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1996278/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1987074/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1704986/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:52859/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:1336958/1‑43 (MQ=25)
ggggCATTGATCGTTCATCCAATCACGCCGCAACCAATTGGCg > 1:887807/1‑43 (MQ=25)
gTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTg > 1:2452893/1‑69 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:495672/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2538834/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:3362413/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:3286217/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2577322/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2517129/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:229445/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1890136/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1700683/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1603909/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1570382/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1483324/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1480041/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1429643/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1357580/69‑1 (MQ=255)
aTCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1153427/69‑1 (MQ=255)
tCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:2001810/68‑1 (MQ=255)
tCACGCCGCAACCAATTGGCGTAGTGACGATTTATCCGGGGATTTCTGCTGACGTCGTGCGCAAtttt < 1:1641983/68‑1 (MQ=255)
|
TTCGTCGTTTGAATACGCCACCCGCCCCGCACGGTGAAGGGGAATTAATCGTTCATCCAATCACCCCACAACCAATTGGCGTAGTGACGATTTATCCAGGGATTTCTGCTGACGTCGTGCGCAATTTT > minE/1179110‑1179237
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
