Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,222,892 |
T→C |
43.5% |
G489G (GGT→GGC) |
yebT → |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,222,892 | 0 | T | C | 43.5%
| 15.9
/ 66.7
| 85 | G489G (GGT→GGC) | yebT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (12/36); new base C (14/23); total (26/59) |
| Fisher's exact test for biased strand distribution p-value = 2.40e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01 |
TTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTT > minE/1222827‑1222952
|
tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgag > 1:2124943/1‑69 (MQ=255)
tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTgag > 1:1692588/1‑69 (MQ=255)
tttCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTga > 1:2008975/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:3327919/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2619194/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2393636/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2912283/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2927065/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:2988393/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1905203/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:157121/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:144889/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1441117/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:732136/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1075892/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:1170015/1‑68 (MQ=255)
gTGCCAGCGCCTCAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGaaa > 1:804513/1‑68 (MQ=255)
aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:2502480/1‑69 (MQ=255)
aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:388387/1‑69 (MQ=255)
aGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGc > 1:1289915/1‑69 (MQ=255)
cataaTGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1060533/57‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGTCGATAAAGGCGAGATGAAAGCCa < 1:1990151/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1307341/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1442937/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2418095/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1553681/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2971013/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2014779/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:1340909/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2992223/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:3182979/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:3319001/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCGATAAACGCGAGATGAAAGCCa < 1:122040/60‑1 (MQ=255)
cAGAATGGATCAATGGCGGAATACGTATTCTGCCGGGCCATAAAGGCGAGATGAAAGCCa < 1:2620653/60‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAGGCCAGCTATCCACTg < 1:1398691/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:3390389/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:308074/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2954673/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:844736/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:992538/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2821099/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2621188/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:556369/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1075718/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:587139/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1897147/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1574888/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1685291/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1547393/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:1396870/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2053302/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2100379/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2129709/68‑1 (MQ=255)
aaTGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTg < 1:2257479/68‑1 (MQ=255)
ggATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTAt > 1:1759572/1‑68 (MQ=255)
cAATGGCGGAATACGTATTCTGCCGGGCGATAAAGGCGAGATGAAAGCCa < 1:2158091/50‑1 (MQ=39)
tGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCAAGCTATCCACTGTAt > 1:1865580/1‑61 (MQ=255)
tATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAgcgc > 1:1275555/1‑68 (MQ=255)
tATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAgcgc > 1:1835017/1‑68 (MQ=255)
cTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag < 1:3169455/69‑1 (MQ=255)
cTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag > 1:1341745/1‑69 (MQ=255)
cTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag > 1:822310/1‑69 (MQ=255)
tGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGgag < 1:1154799/68‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:744312/67‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:746964/67‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:997172/67‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:964728/67‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:494278/67‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:461018/67‑1 (MQ=255)
gggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAAc < 1:2693277/67‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:3414160/62‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:337841/62‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:2895222/62‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:2762083/62‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:1164580/62‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:912731/62‑1 (MQ=255)
gggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:2321169/62‑1 (MQ=255)
ggCGATATAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGc < 1:3329795/69‑1 (MQ=255)
ggCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGc < 1:414700/69‑1 (MQ=255)
ggCGATAAAGGCGAGATGAAAGCCAGCTATCCACTGTATGCCAATCAGGAAAAAGCGCTgg < 1:807216/61‑1 (MQ=255)
gATAAAGGTGATATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACATCCtt < 1:2311612/69‑1 (MQ=255)
gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:3412889/69‑1 (MQ=255)
gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:1691657/69‑1 (MQ=255)
gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:2238134/69‑1 (MQ=255)
gATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCtt < 1:2866755/69‑1 (MQ=255)
|
TTTCTCGGTGCCAGCGCCTCAGAATGGATCAATGGCGGGATACGTATTCTGCCGGGCGATAAAGGTGAGATGAAAGCCAGCTATCCACTGTATGCCAATTTGGAAAAAGCGCTGGAGAACAGCCTT > minE/1222827‑1222952
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
