Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,251,089 T→C 24.4% Q68Q (CAA→CAG)  ruvC ← component of RuvABC resolvasome, endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,251,0890TC24.4% 129.5 / 37.9 106Q68Q (CAA→CAG) ruvCcomponent of RuvABC resolvasome, endonuclease
Reads supporting (aligned to +/- strand):  ref base T (50/30);  new base C (11/15);  total (61/45)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

ACAATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATG  >  minE/1251024‑1251152
                                                                 |                                                               
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACtt                                                                >  1:2145472/1‑67 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACtt                                                                >  1:784654/1‑67 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:2972533/1‑68 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:3053494/1‑68 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:373592/1‑68 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:1974571/1‑68 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:808838/1‑68 (MQ=255)
acaATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:1953025/1‑68 (MQ=255)
acaATAGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTg                                                               >  1:3377251/1‑68 (MQ=255)
  aaTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc                                                            <  1:2499261/69‑1 (MQ=255)
  aaTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc                                                            <  1:331657/69‑1 (MQ=255)
  aaTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc                                                            <  1:1883307/69‑1 (MQ=255)
   aTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTc                                                            >  1:1827556/1‑68 (MQ=255)
   aTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCa                                                           >  1:1082699/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCa                                                           >  1:798615/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTc                                                            >  1:386459/1‑68 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTc                                                            >  1:177884/1‑68 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:81966/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:3260444/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:1239713/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:2098883/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:2086793/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:145862/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:2949666/1‑69 (MQ=255)
   aTCGCCACGCCGCGCGCCTGCCCCAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCa                                                           >  1:658588/1‑69 (MQ=255)
        cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATgg                                                       >  1:470549/1‑68 (MQ=255)
        cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATgg                                                       >  1:2667372/1‑68 (MQ=255)
        cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATgg                                                       >  1:2977027/1‑68 (MQ=255)
        cACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATg                                                        >  1:474589/1‑67 (MQ=255)
            ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGAGTTGTTCAATGGTGaa                                                   >  1:1296165/1‑68 (MQ=255)
            ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa                                                   >  1:2191210/1‑68 (MQ=255)
            ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa                                                   >  1:887554/1‑68 (MQ=255)
            ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa                                                   >  1:885634/1‑68 (MQ=255)
            ccgcGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGaa                                                   >  1:1201137/1‑68 (MQ=255)
                gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata                                                >  1:2168732/1‑67 (MQ=255)
                gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata                                                >  1:817470/1‑67 (MQ=255)
                gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata                                                >  1:1784721/1‑67 (MQ=255)
                gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata                                                >  1:2346462/1‑67 (MQ=255)
                gcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAaata                                                >  1:2537255/1‑67 (MQ=255)
                      gccccAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa                                                          <  1:497222/49‑1 (MQ=255)
                        cccAGTTTCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa                                                          <  1:16888/49‑1 (MQ=255)
                         ccAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTgg                                      <  1:410884/68‑1 (MQ=255)
                         ccAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTgg                                      <  1:3270871/68‑1 (MQ=255)
                         ccAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTgg                                      <  1:1801997/68‑1 (MQ=255)
                              ttCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa                                                          <  1:220693/43‑1 (MQ=38)
                              ttCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:3253014/45‑1 (MQ=255)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCCTAAAGACCTGTTCAATg                                                        <  1:3072755/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:2211311/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:2962993/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:3005390/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:2358009/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:2333987/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:2240489/44‑1 (MQ=38)
                               tCAGGGCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:1500478/44‑1 (MQ=38)
                                   ggCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCAATg                                                        <  1:2677905/40‑1 (MQ=38)
                                    gCTGAGTCAGCGTTCTTCGCCATAAAGACCTGTTCaa                                                          <  1:2789511/37‑1 (MQ=38)
                                       gAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGAtt                       <  1:2426511/69‑1 (MQ=255)
                                       gAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGAtt                       <  1:1681497/69‑1 (MQ=255)
                                       gAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGAtt                       <  1:758639/69‑1 (MQ=255)
                                            aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:3271125/60‑1 (MQ=255)
                                            aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:3149686/60‑1 (MQ=255)
                                            aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:778089/60‑1 (MQ=255)
                                            aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:611052/60‑1 (MQ=255)
                                            aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1190157/60‑1 (MQ=255)
                                            aGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:2918797/60‑1 (MQ=255)
                                               gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc               <  1:1371446/69‑1 (MQ=255)
                                               gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc               <  1:1374081/69‑1 (MQ=255)
                                               gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc               <  1:1734260/69‑1 (MQ=255)
                                               gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc               <  1:1518814/69‑1 (MQ=255)
                                               gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc               <  1:2211864/69‑1 (MQ=255)
                                               gTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCAc               <  1:2071545/69‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCTAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:1809359/1‑69 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:507235/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:3011819/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:523404/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:569366/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1585738/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1556332/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:1510743/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1334456/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1290739/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:1218396/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:912222/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1855416/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:503457/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:3010831/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:1924926/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:1985489/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:2243773/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:2267672/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:3013022/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:2429069/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           <  1:2814541/44‑1 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGtgat                           >  1:289695/1‑44 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:941002/1‑69 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:2908817/1‑69 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:1354287/1‑69 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:2393651/1‑69 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:1911502/1‑69 (MQ=255)
                                                            aaGACTTGTTCAATGGCGAAATAATCAGGCTGGAAATGGGTGATGATTTCCGTCACGCCCGCATAGATg  >  1:601604/1‑69 (MQ=255)
                                                                 |                                                               
ACAATCGCCACGCCGCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATG  >  minE/1251024‑1251152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A