Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,257,879 |
G→A |
58.2% |
A174A (GCG→GCA) |
yecP → |
predicted methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,257,879 | 0 | G | A | 58.2%
| 10.2
/ 29.7
| 43 | A174A (GCG→GCA) | yecP | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (6/12); new base A (12/13); total (18/25) |
| Fisher's exact test for biased strand distribution p-value = 3.68e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 |
GTGCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTC > minE/1257831‑1257941
|
gtgCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTa < 1:3223495/68‑1 (MQ=255)
gtgCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTa < 1:2620751/68‑1 (MQ=255)
gtgCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTa < 1:1798089/68‑1 (MQ=255)
gtgCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTa < 1:2002597/68‑1 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTTTTACCGTTAGGt > 1:82431/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:1260617/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:3156403/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:2908251/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:520903/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:524883/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:227375/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:785011/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:849036/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:91491/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:1769885/1‑66 (MQ=255)
tgCCAGTTTGAAGCAGTGCGTAAACTGCTGGGTAACGATCAGCGCGCACATTTGTTACCGTTAGGt > 1:1505485/1‑66 (MQ=255)
tGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAgg < 1:13696/57‑1 (MQ=255)
tGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACt > 1:3287905/1‑69 (MQ=255)
tGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACt > 1:475724/1‑69 (MQ=255)
aaaCTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTa < 1:1134256/46‑1 (MQ=255)
gggTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCttt < 1:1362677/67‑1 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGa > 1:2023828/1‑39 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGAt < 1:1647354/67‑1 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGAt < 1:1598106/67‑1 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGAt < 1:1962775/67‑1 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGAt < 1:640055/67‑1 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGAt < 1:721607/67‑1 (MQ=255)
tAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATAc > 1:3082598/1‑69 (MQ=255)
aTCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCt > 1:3394148/1‑69 (MQ=255)
aTCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCt > 1:3271603/1‑69 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:543117/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:557763/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:96569/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:3231875/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:2884370/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:2739047/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:2543278/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:243650/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:2164488/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:2127516/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:191607/56‑1 (MQ=255)
cAGCGCGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:1875335/56‑1 (MQ=255)
cAGCGAGCACATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCtt < 1:2930588/56‑1 (MQ=255)
cgcgcgcATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTc < 1:3144619/68‑1 (MQ=255)
cgcgcgcATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTc < 1:2520542/68‑1 (MQ=255)
cgcgcgcATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTc < 1:2454179/68‑1 (MQ=255)
cgcgcgcATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTc < 1:1742155/68‑1 (MQ=255)
cgcATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGAt < 1:2977356/53‑1 (MQ=255)
|
GTGCCAGTTTGAAGCGGTGCGTAAACTACTGGGTAATGATCAGCGCGCGCATTTGTTACCGTTAGGTATTGAACAACTTCCGGCACTGAAAGCCTTTGATACCGTCTTTTC > minE/1257831‑1257941
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
