Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,258,176 G→A 65.5% A273A (GCG→GCA)  yecP → predicted methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,258,1760GA65.5% 54.4 / 45.5 81A273A (GCG→GCA) yecPpredicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (15/13);  new base A (28/25);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

TCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAA  >  minE/1258109‑1258233
                                                                   |                                                         
tCATCCCTTCCGCGCTGTCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:1144114/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGTCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:1148431/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCgg                                                          >  1:841307/1‑69 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:2211769/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:578358/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:3267042/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:1663097/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:1749325/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:1751468/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:2186316/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:2208951/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:954042/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:2872170/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:2332625/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:246976/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:2731581/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:2622416/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:916352/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:1189946/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:1254615/68‑1 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           >  1:1355772/1‑68 (MQ=255)
tCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCg                                                           <  1:141680/68‑1 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:2701633/1‑67 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:2447113/1‑67 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:2186673/1‑67 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:291224/1‑67 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:647966/1‑67 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:1574141/1‑67 (MQ=255)
    cccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGAt                                                        >  1:1574441/1‑67 (MQ=255)
     ccTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTT‑TAGATATCCGCATTGCGGATGTGa                                                    >  1:1231278/1‑69 (MQ=255)
                ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTaccac                                          <  1:3043737/69‑1 (MQ=255)
                ggcgctgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTaccac                                          <  1:1616151/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTTCCACCACag                                     <  1:1164376/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag                                     <  1:594581/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag                                     <  1:2344461/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag                                     <  1:45969/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag                                     <  1:1001838/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag                                     <  1:2599921/69‑1 (MQ=255)
                     tgAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACag                                     <  1:2708783/69‑1 (MQ=255)
                         aaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaag                                  <  1:280878/68‑1 (MQ=255)
                         aaaCTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACagaag                                  <  1:2615551/68‑1 (MQ=255)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGAtgt                                                      >  1:2535987/1‑36 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGa                                                    >  1:913293/1‑38 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:508194/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2856284/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:3368350/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:916260/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2855889/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2953776/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:301624/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:3034774/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:3067803/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:3071854/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:867771/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2560424/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:3309967/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2702561/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:3158133/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1034629/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1622627/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1766674/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1794134/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1566073/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1993413/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:1137297/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2327393/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2356394/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:253146/1‑39 (MQ=25)
                                     gaagTGTGGTTTTGTTGATATTCGCATTGCAGATGTGAg                                                   >  1:2605234/1‑39 (MQ=25)
                                          gtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGagc                               >  1:1817635/1‑54 (MQ=255)
                                          gtgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGaa                 >  1:1943760/1‑68 (MQ=255)
                                           tgGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAatg               <  1:549819/69‑1 (MQ=255)
                                                       tatCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCg    <  1:3255424/68‑1 (MQ=255)
                                                        atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa  >  1:126385/1‑69 (MQ=255)
                                                        atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa  >  1:932661/1‑69 (MQ=255)
                                                        atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa  >  1:3376728/1‑69 (MQ=255)
                                                        atCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGaa  >  1:1738407/1‑69 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACGACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:2695153/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCTAATGGATGGt         <  1:483190/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1502546/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:2680982/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:2534152/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1049225/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1088630/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:2883288/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:895612/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:2051549/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1375008/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:759638/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:686964/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1425301/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1643787/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1856911/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:3134164/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1579243/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:475263/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:409286/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:3383082/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1633192/54‑1 (MQ=255)
                                                                tGCAGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGt         <  1:1638725/54‑1 (MQ=255)
                                                                   |                                                         
TCATCCCTTCCGCGCTGGCGCTGAAAAACTGGCTGAAGAAGTGTGGTTTTGTAGATATCCGCATTGCGGATGTGAGCGTTACCACCACAGAAGAGCAGCGACGCACCGAATGGATGGTTACCGAA  >  minE/1258109‑1258233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A