Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,307,479 |
A→T |
25.2% |
T39T (ACT→ACA) |
yeeF ← |
predicted amino‑acid transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,307,479 | 0 | A | T | 25.2%
| 157.8
/ 54.6
| 103 | T39T (ACT→ACA) | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base A (46/31); new base T (8/18); total (54/49) |
| Fisher's exact test for biased strand distribution p-value = 1.30e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 |
Only 100 of 104 total aligned reads displayed.
AATGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACTAACGTAAGCG > minE/1307420‑1307546
|
aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:2225833/1‑69 (MQ=255)
aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:2255075/1‑69 (MQ=255)
aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:2923834/1‑69 (MQ=255)
aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:468249/1‑69 (MQ=255)
aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:3245022/1‑69 (MQ=255)
aatGCGAACGCATAGGCTGTCGGCACATGACAATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:3006226/1‑69 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:921875/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2351115/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2410855/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2968281/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:1783177/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:3097665/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:1496004/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:901511/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:786572/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:1429440/65‑1 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:1949770/1‑69 (MQ=255)
ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2900921/1‑69 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:2801190/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:230278/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:2672928/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:1803583/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:522120/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:502556/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:158885/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:3192961/69‑1 (MQ=255)
cTGTCGGCACATGACCATCCGTGAGGCATGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:273480/69‑1 (MQ=255)
gTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2146470/61‑1 (MQ=255)
gTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCata < 1:826282/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:689245/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:617620/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:3389029/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:3031242/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2491353/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2335029/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1146408/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2196574/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1783170/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1420904/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1307545/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1248460/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2063548/69‑1 (MQ=255)
cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2126599/69‑1 (MQ=255)
acaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2326851/68‑1 (MQ=255)
acaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1594175/68‑1 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2382222/1‑58 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2073247/1‑58 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:1767450/1‑58 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2595928/1‑58 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:743491/1‑58 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2137171/1‑58 (MQ=255)
caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:480122/1‑58 (MQ=255)
aTGACCATCCGTGAGGCCTGGAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:431811/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAGAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1873203/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCaa > 1:1530308/1‑68 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:2141461/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:3067466/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1587005/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:149227/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1460015/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:63199/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:943732/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:945860/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:2767078/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1784245/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1880109/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:245417/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:2966399/1‑69 (MQ=255)
aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:187048/1‑69 (MQ=255)
aTGACAATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAg > 1:2056712/1‑64 (MQ=255)
tCCGTGAGGCCTGAGACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:3076209/1‑52 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGc > 1:381954/1‑51 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:1894492/1‑52 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:3212540/1‑52 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:3235876/1‑52 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:918384/1‑52 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:2002942/1‑52 (MQ=255)
tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:868918/1‑52 (MQ=255)
cGTGAGGCCTGAAACGATCCCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatcat > 1:883259/1‑69 (MQ=255)
cGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatcat > 1:2861686/1‑69 (MQ=255)
cGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatcat > 1:1952544/1‑69 (MQ=255)
cGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatca > 1:688854/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:947922/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:1004414/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2421852/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:724133/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2993234/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:1443504/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2972846/1‑68 (MQ=255)
cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2983064/1‑68 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:661373/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:763755/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:2863784/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:3384804/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:3347788/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:1531849/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:297996/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATAATTACAACCGGAACt < 1:1479152/69‑1 (MQ=255)
aCGATACCAAAAGTATCAAACAGCGGCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:344161/69‑1 (MQ=255)
aaGTATCAAACAGCGTCATCGCCTGCATATAGGCAAGACCCATCATTACAACCGGAACTAACGTAAGCg < 1:1415702/69‑1 (MQ=255)
|
AATGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACTAACGTAAGCG > minE/1307420‑1307546
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
