Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,314,264 |
T→C |
50.6% |
R173R (CGT→CGC) |
hisB → |
fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,314,264 | 0 | T | C | 50.6%
| ‑2.2
/ 71.5
| 79 | R173R (CGT→CGC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (29/10); new base C (21/19); total (50/29) |
| Fisher's exact test for biased strand distribution p-value = 6.19e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01 |
CAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAAC > minE/1314212‑1314330
|
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2531587/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1767672/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1808150/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1850906/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2022661/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2200629/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2372258/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2428536/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2457868/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1176008/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:25853/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2756915/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:3071039/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:320169/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:405993/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:675360/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:842111/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:993568/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1004565/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1294335/3‑68 (MQ=255)
cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1351958/3‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTATGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:2625292/1‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:844075/1‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:506822/1‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:467611/1‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1717406/1‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1546485/1‑68 (MQ=255)
gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:3278024/1‑68 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGTTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2157673/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2827078/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2890087/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:285864/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1401765/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3119884/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3427525/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:36162/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:384040/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:392819/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:463524/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:836983/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:843167/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2783626/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2248628/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1695059/1‑69 (MQ=255)
aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1514384/1‑69 (MQ=255)
tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:2177280/1‑69 (MQ=255)
tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1550899/1‑69 (MQ=255)
tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1570672/1‑69 (MQ=255)
tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:645457/1‑69 (MQ=255)
aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2142160/69‑1 (MQ=255)
aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:3198566/69‑1 (MQ=255)
aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:220111/69‑1 (MQ=255)
aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:899019/69‑1 (MQ=255)
aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTCACGTTc < 1:435799/69‑1 (MQ=255)
cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATGCCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1304822/69‑1 (MQ=255)
cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2886370/69‑1 (MQ=255)
cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1397864/69‑1 (MQ=255)
cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2858445/69‑1 (MQ=255)
cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2246130/69‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCTCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1715190/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAATAGACGCAAATTGACGTCCAGGTGTGGCt < 1:978431/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2865435/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2791658/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1993443/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2000525/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2014797/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2255749/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:596153/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1575179/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2946927/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3038616/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1542223/66‑1 (MQ=255)
taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3122365/66‑1 (MQ=255)
aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1460387/66‑1 (MQ=255)
aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:914324/66‑1 (MQ=255)
aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:429962/66‑1 (MQ=255)
gTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2562875/58‑1 (MQ=255)
aCGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:808087/55‑1 (MQ=255)
cGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAac > 1:1407849/1‑69 (MQ=255)
|
CAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAAC > minE/1314212‑1314330
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
