Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,314,660 |
C→T |
24.6% |
L305L (CTC→CTT) |
hisB → |
fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,314,660 | 0 | C | T | 24.6%
| 123.1
/ 32.6
| 97 | L305L (CTC→CTT) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (47/26); new base T (11/13); total (58/39) |
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 |
GAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGATCATC > minE/1314595‑1314715
|
gAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCa < 1:310515/69‑1 (MQ=255)
tttACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATac > 1:701701/1‑69 (MQ=255)
tttACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATac > 1:2292637/1‑69 (MQ=255)
ttaacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATac > 1:1461699/4‑69 (MQ=255)
tacctaccAGCGCGTGGGGGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:1295359/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGctct < 1:346804/62‑1 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2325396/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:1906631/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2100395/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:1411684/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2115493/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2287082/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2390391/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2467964/1‑69 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac < 1:2519250/69‑1 (MQ=255)
tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2602999/1‑69 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:1020409/68‑1 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:2630644/68‑1 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:1861729/68‑1 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:3046647/68‑1 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:16866/68‑1 (MQ=255)
cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:1613683/68‑1 (MQ=255)
taccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCAt > 1:1325888/1‑68 (MQ=255)
taccAGCGCGTGGGCGATCTCAGCACCGAAAAGAACGAGCACTTCTTCCGATCGCTCTCATACACCATg > 1:1980713/1‑69 (MQ=255)
accAGCGCTTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:2906739/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:1631995/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:107856/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:23920/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:81620/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:3279815/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:2993358/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:678700/1‑69 (MQ=255)
accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:1728738/1‑69 (MQ=255)
aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:2092041/62‑1 (MQ=255)
aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:460720/1‑68 (MQ=255)
aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:252309/1‑68 (MQ=255)
aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:1725017/1‑68 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACg > 1:2926639/1‑68 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:2428061/1‑69 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:2829735/1‑69 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:3307337/1‑69 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:2327383/1‑69 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:953520/1‑69 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:718420/1‑69 (MQ=255)
tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:1380635/1‑69 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:1010773/59‑1 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:3382371/59‑1 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:2337218/59‑1 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:70166/59‑1 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:855511/59‑1 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:1491344/59‑1 (MQ=255)
ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:186985/59‑1 (MQ=255)
gCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:952533/58‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:2891237/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:3222942/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:1026886/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:917852/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:1326324/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:2289844/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:187208/69‑1 (MQ=255)
aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:154612/69‑1 (MQ=255)
aTCGAGCACTGCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:492390/69‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:600721/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:640459/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:756108/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:3232289/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:3226409/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:958478/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2239827/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:1092663/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:128007/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:1541444/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2227285/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2505487/68‑1 (MQ=255)
gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2993713/68‑1 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:2836492/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:3111404/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:1300758/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:1332162/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:1437212/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:733557/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:178172/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:2929457/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:2535184/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:3374266/1‑65 (MQ=255)
tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGa > 1:31965/1‑64 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACCCCTGAAAACCAAAGGTAAAAACGatcatc > 1:609244/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:566816/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:3140858/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:546955/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:2342740/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:1666756/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:3048015/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:150423/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:3292324/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:1105213/1‑69 (MQ=255)
tcttcCGTTCGCTCTCATACACCAAGGGCGAGACGCTACACCTTAAAACCAAAGGTAAAAACGa > 1:1254214/1‑64 (MQ=255)
tcttcAGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:2684736/1‑69 (MQ=255)
|
GAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGATCATC > minE/1314595‑1314715
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
