Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,322,844 T→C 32.6% P30P (CCA→CCG)  galF ← predicted subunit with GalU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,322,8440TC32.6% 79.7 / 54.1 101P30P (CCA→CCG) galFpredicted subunit with GalU
Reads supporting (aligned to +/- strand):  ref base T (7/61);  new base C (19/14);  total (26/75)
Fisher's exact test for biased strand distribution p-value = 9.13e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 107 total aligned reads displayed.

TTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAT  >  minE/1322780‑1322912
                                                                |                                                                    
tttCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGt                                                                   >  1:3018617/1‑68 (MQ=255)
tttCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGt                                                                   >  1:3151088/1‑68 (MQ=255)
  tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                                                                <  1:281044/69‑1 (MQ=255)
  tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                                                                <  1:2717993/69‑1 (MQ=255)
  tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                                                                <  1:2357085/69‑1 (MQ=255)
  tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                                                                <  1:1517419/69‑1 (MQ=255)
  tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCCACGATTGGTAGc                                                                <  1:500841/69‑1 (MQ=255)
  tCTTTGATCCCTGCAGCCACAATCTCATCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                                                                <  1:955442/69‑1 (MQ=255)
   cTTTGATCCCTGCATCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc                                                                <  1:2537936/68‑1 (MQ=255)
        aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct                                                            <  1:1906946/67‑1 (MQ=255)
        aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct                                                            <  1:932872/67‑1 (MQ=255)
        aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct                                                            <  1:1043551/67‑1 (MQ=255)
        aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATCGGCTTGTCGACGATTGGTAGCAtct                                                            <  1:334774/67‑1 (MQ=255)
        aTCCCTGCAGCCACAATCTCATCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct                                                            <  1:128591/67‑1 (MQ=255)
                   cacaATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc                                               <  1:1522053/69‑1 (MQ=255)
                   cacaATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc                                               <  1:623961/69‑1 (MQ=255)
                   cacaATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc                                               <  1:3142137/69‑1 (MQ=255)
                   cacaATCTCGTCAACAATGTACTGAATCATTGGATTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc                                               <  1:8075/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:2858214/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:2132728/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:855889/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:417349/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:118408/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:2637155/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:181540/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:1268559/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:162715/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:3052536/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:1234184/69‑1 (MQ=255)
                      aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt                                            <  1:3048215/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:2771730/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:247057/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:3194939/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:2396689/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:2909348/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:2178689/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:1265464/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:38032/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:810895/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:796206/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa                                           <  1:1825474/69‑1 (MQ=255)
                       aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGCAATCGCCTTa                                           <  1:3096873/69‑1 (MQ=255)
                          tcGTCAACAATGTACTTAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:1305189/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTTGTAGCATCTCTTTggg                                                     <  1:3276158/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:2340839/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:100249/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:3032487/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:423569/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:18846/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:1456931/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg                                                     <  1:1683604/56‑1 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGt                                         >  1:3341716/1‑68 (MQ=255)
                          tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGAATGGTAGCATCTCTTTggg                                                     <  1:1759437/56‑1 (MQ=255)
                             tCAACGATGTACTGAATCATTGGGTTGTCGACGATCgg                                                                    >  1:3250244/1‑38 (MQ=25)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:739077/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:841078/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:887270/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:3181401/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:103793/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:404728/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:1905889/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:2320023/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:2216766/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:1939888/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:1634350/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:1207277/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:108491/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:2854158/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:1079180/1‑38 (MQ=38)
                             tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg                                                                    >  1:2970916/1‑38 (MQ=38)
                                    tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:2646045/69‑1 (MQ=255)
                                    tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:3172024/69‑1 (MQ=255)
                                    tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:2460047/69‑1 (MQ=255)
                                    tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:3246647/69‑1 (MQ=255)
                                    tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:358037/69‑1 (MQ=255)
                                    tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:2408776/69‑1 (MQ=255)
                                     gTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:1962361/68‑1 (MQ=255)
                                     gTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:2610437/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:3299558/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:2977602/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:2443642/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:2819806/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:1210240/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:130591/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:1881058/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:1830757/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:742671/68‑1 (MQ=255)
                                        cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATg                          >  1:2823753/1‑69 (MQ=255)
                                             tCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATc                      >  1:971315/1‑68 (MQ=255)
                                             tCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATc                      >  1:19192/1‑68 (MQ=255)
                                             tCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATc                      >  1:324988/1‑68 (MQ=255)
                                               aTTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:1099695/58‑1 (MQ=255)
                                                          gacgaTTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat                           <  1:908149/50‑1 (MQ=255)
                                                            cgaTTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa                              <  1:688100/45‑1 (MQ=255)
                                                                tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt  <  1:1558718/69‑1 (MQ=255)
                                                                tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt  <  1:1493053/69‑1 (MQ=255)
                                                                tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt  <  1:3154291/69‑1 (MQ=255)
                                                                tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt  <  1:406049/69‑1 (MQ=255)
                                                                tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt  <  1:2958647/69‑1 (MQ=255)
                                                                tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt  <  1:2358458/69‑1 (MQ=255)
                                                                |                                                                    
TTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAT  >  minE/1322780‑1322912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A