Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,381,097 |
C→T |
24.2% |
A300A (GCG→GCA) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,381,097 | 0 | C | T | 24.2%
| 49.6
/ 10.5
| 41 | A300A (GCG→GCA) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (9/22); new base T (4/6); total (13/28) |
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCA > minE/1381031‑1381160
|
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:95189/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:2554425/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:232939/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:2964201/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:3209890/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:404340/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:188631/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:1683343/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:1446894/69‑1 (MQ=255)
aaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGc < 1:1158188/69‑1 (MQ=255)
gCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa > 1:590054/1‑68 (MQ=255)
gCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa > 1:923349/1‑68 (MQ=255)
cAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAg > 1:1341603/1‑68 (MQ=255)
cAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAg > 1:2109197/1‑68 (MQ=255)
aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:742659/69‑1 (MQ=255)
aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:3423196/69‑1 (MQ=255)
aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:1130118/69‑1 (MQ=255)
aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:1080308/69‑1 (MQ=255)
aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:2213161/69‑1 (MQ=255)
aGCGTTTGCAATAGATAGTTGGTCAGTGCCATCCGTCCGACACAGGCGATTGCCAGCACCAGCTTAAAg < 1:171347/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:2338805/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:987612/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:860887/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:707166/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:619192/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:593365/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:3244820/69‑1 (MQ=255)
tttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:3232771/69‑1 (MQ=255)
ttGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:1271162/68‑1 (MQ=255)
agatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:387978/1‑69 (MQ=255)
agatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:1928512/1‑69 (MQ=255)
agatagTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:2345604/1‑69 (MQ=255)
agatagGTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGc > 1:440677/1‑69 (MQ=255)
ttGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGc < 1:2702866/55‑1 (MQ=255)
cAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGc < 1:364233/68‑1 (MQ=255)
aGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGc < 1:778631/67‑1 (MQ=255)
cgtccgACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGc > 1:378523/1‑69 (MQ=255)
gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:1988465/1‑69 (MQ=255)
gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:2047514/1‑69 (MQ=255)
gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:1480871/1‑69 (MQ=255)
gCGATTGCCAGCACCAGCTTAAAGCGGCTTAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCa > 1:2981246/1‑69 (MQ=255)
|
AAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCA > minE/1381031‑1381160
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
