Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,381,675 |
A→G |
55.6% |
L108L (TTG→CTG) |
yeiB ← |
conserved inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,381,675 | 0 | A | G | 55.6%
| 3.8
/ 26.6
| 36 | L108L (TTG→CTG) | yeiB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (4/12); new base G (14/6); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.76e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 |
GCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAC > minE/1381607‑1381742
|
gCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCaa < 1:1147437/69‑1 (MQ=255)
gATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGcc < 1:780150/68‑1 (MQ=255)
gATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGcc < 1:3127115/68‑1 (MQ=255)
gATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGcc < 1:298354/68‑1 (MQ=255)
gcgcACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAAc > 1:1454478/1‑67 (MQ=255)
gcgcACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACa > 1:1754269/1‑68 (MQ=255)
aaGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCca > 1:2578813/1‑67 (MQ=255)
aaGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCca > 1:174498/1‑67 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa < 1:1238482/68‑1 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacaa < 1:2854311/68‑1 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:3372201/69‑1 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:2131435/69‑1 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:2771183/69‑1 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:3406825/69‑1 (MQ=255)
cccACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg < 1:680495/69‑1 (MQ=255)
accaGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACt < 1:617227/69‑1 (MQ=255)
accaGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACt < 1:2354007/69‑1 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2526983/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2255182/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:290880/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:785878/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:797832/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1631546/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1149027/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:134007/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1381123/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1481970/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:2515294/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:1828850/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACt > 1:239462/1‑68 (MQ=255)
ccaGCCCGTAAGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAAAAAAGCCCAGCAAGACt > 1:672072/1‑68 (MQ=255)
aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCTTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1428743/69‑1 (MQ=255)
aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:164796/69‑1 (MQ=255)
aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1572003/69‑1 (MQ=255)
aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1840454/69‑1 (MQ=255)
aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1857597/69‑1 (MQ=255)
aaGCCAGCAGAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAGCAAGACTAACAGCGTTa < 1:1175429/69‑1 (MQ=255)
aaaaTATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAc < 1:2958829/69‑1 (MQ=255)
aaaaTATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAAAAAGACTAACAGCGTTAACCGCGAc < 1:1659355/69‑1 (MQ=255)
|
GCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAC > minE/1381607‑1381742
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
