Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,417,268 T→G 22.8% A487A (GCT→GCG)  yejM → predicted hydrolase, inner membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,417,2680TG22.8% 187.0 / 62.4 127A487A (GCT→GCG) yejMpredicted hydrolase, inner membrane
Reads supporting (aligned to +/- strand):  ref base T (52/46);  new base G (27/2);  total (79/48)
Fisher's exact test for biased strand distribution p-value = 5.30e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 132 total aligned reads displayed.

CCACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCT  >  minE/1417202‑1417331
                                                                  |                                                               
ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct                                                               <  1:1085954/69‑1 (MQ=255)
ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct                                                               <  1:1758894/69‑1 (MQ=255)
ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct                                                               <  1:627319/69‑1 (MQ=255)
ccACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGctct                                                               <  1:2414390/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:1065495/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:2035085/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:2103992/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:231660/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:2366171/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:1622499/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:2470841/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:2488478/69‑1 (MQ=255)
       cATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGAt                                                        <  1:3278428/69‑1 (MQ=255)
           tGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATa                                                    >  1:1065854/1‑69 (MQ=255)
               ggTGCCATTAGTGATTCACTGGCCAGGCACCCCGGCGCAGCGTATTAATGCGCTGACTGATCATACCg                                                 <  1:801570/68‑1 (MQ=255)
                  gCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCt                                             >  1:896104/1‑69 (MQ=255)
                  gCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCt                                             >  1:2942133/1‑69 (MQ=255)
                  gCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCt                                             >  1:2349240/1‑69 (MQ=255)
                        tctGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:570603/67‑1 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCtgatga                                        >  1:433899/1‑68 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCtgatg                                         >  1:3062285/1‑67 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:944200/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:213132/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:80991/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:598787/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:3425316/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:2974719/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:3009941/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:3120695/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:1686274/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:158417/1‑69 (MQ=255)
                        aGTGATTCACTGGCCAGGCACGCCGGCACAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:1755416/1‑69 (MQ=255)
                            aTTCACTGGCCAGGCAGGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:2141742/1‑65 (MQ=255)
                            aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:253339/1‑65 (MQ=255)
                            aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:555421/1‑65 (MQ=255)
                            aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:1652682/1‑65 (MQ=255)
                            aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:3392039/1‑65 (MQ=255)
                            aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       >  1:1171289/1‑65 (MQ=255)
                            aTTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACAGATCTGATgac                                       >  1:3155422/1‑65 (MQ=255)
                              tCACTGGCCAGGCACGCCGTCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:1135766/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:1454941/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:1316895/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:3236348/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:3235310/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:2334970/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:1356121/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:1241030/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:611996/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATgac                                       <  1:2265288/63‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGc                                  >  1:735773/1‑68 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 <  1:736390/69‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:1845909/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:1847387/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 <  1:855771/69‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:1921884/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 <  1:3259448/69‑1 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:1180761/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:773489/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:367897/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:1316768/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:1595440/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 >  1:2399827/1‑69 (MQ=255)
                              tCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 <  1:2113232/69‑1 (MQ=255)
                               cACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 <  1:1525128/68‑1 (MQ=255)
                               cACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCt                                 <  1:1672630/68‑1 (MQ=255)
                                         gCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGc                            <  1:2587790/63‑1 (MQ=255)
                                         gCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGcc                      <  1:1404433/69‑1 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 >  1:1049596/1‑69 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 <  1:2000115/69‑1 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 <  1:796700/69‑1 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 >  1:1824291/1‑69 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 >  1:32876/1‑69 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 >  1:2441657/1‑69 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 <  1:3355230/69‑1 (MQ=255)
                                              ccGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 <  1:1532313/69‑1 (MQ=255)
                                              caggCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTa                 <  1:708126/67‑1 (MQ=255)
                                                    cAGCGTATTAATGCTCTGACTGATCATACCGTTCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           <  1:268521/69‑1 (MQ=255)
                                                        gTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGca       <  1:2429521/69‑1 (MQ=255)
                                                        gTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGca       <  1:1218875/69‑1 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:698357/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:1515857/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:186068/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:188124/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:290908/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:2763979/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:263002/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:638870/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:2106003/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:2227875/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:132160/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:1843417/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:429030/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:370250/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCAGCTACATGTc           >  1:2008924/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGATCTGAAGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:2737055/1‑63 (MQ=255)
                                                          aTTAATGCGCTGACTGATCATACCGACCTGATGACGACGCTGATGCAACGCCTGCTACATGTc           >  1:2605221/1‑63 (MQ=255)
                                                              aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt  >  1:3339245/1‑68 (MQ=255)
                                                              aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt  >  1:1814431/1‑68 (MQ=255)
                                                              aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCt  >  1:1895239/1‑68 (MQ=255)
                                                              aTGCTCTGACTGATCATACCGATCTGATGACGACGCTGATCCAACGCCTGc                   >  1:1288267/1‑51 (MQ=255)
                                                                  |                                                               
CCACGGTCATCTGCAGGTGCCATTAGTGATTCACTGGCCAGGCACGCCGGCGCAGCGTATTAATGCTCTGACTGATCATACCGATCTGATGACGACGCTGATGCAACGCCTGCTACATGTCAGCACACCT  >  minE/1417202‑1417331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A