Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,436,912 G→A 40.5% A349V (GCT→GTT)  ada ← fused DNA‑binding transcriptional dual regulator and O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,436,9120GA40.5% 49.0 / 73.1 106A349V (GCT→GTT) adafused DNA‑binding transcriptional dual regulator and O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (21/42);  new base A (15/28);  total (36/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 110 total aligned reads displayed.

AGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCAC  >  minE/1436850‑1436978
                                                              |                                                                  
aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:926747/69‑1 (MQ=255)
aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:1721146/69‑1 (MQ=255)
aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:1564371/69‑1 (MQ=255)
aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:1545036/69‑1 (MQ=255)
aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:2402559/69‑1 (MQ=255)
aGTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:896810/69‑1 (MQ=255)
 gTGGTTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTcgc                                                              <  1:3388113/68‑1 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:564787/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:55286/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:1196742/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:1243607/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:2706456/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:874032/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgcgg                                                            >  1:2340701/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCgagg                                                            >  1:2465465/1‑67 (MQ=255)
    gCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTCCAGCTTCgcgg                                                            >  1:2733808/1‑67 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:2606166/69‑1 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:1033566/69‑1 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:1727625/69‑1 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:20014/69‑1 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:895691/69‑1 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:841323/69‑1 (MQ=255)
               ggTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTgcg                                               <  1:704778/69‑1 (MQ=255)
                gTTCATCATCGGCAAACAGATCAAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1016837/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGTCGCAGCAGTTgcgc                                              <  1:870839/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:2181022/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:2175309/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:2276826/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:2004253/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1455167/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:2498408/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:185261/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1843001/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:3240139/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1181093/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1740300/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:651227/69‑1 (MQ=255)
                gTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:862711/69‑1 (MQ=255)
                 ttCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1552266/68‑1 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:3332310/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:1187092/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:2963488/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:2847750/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:1430187/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:659904/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:2649034/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:1589204/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:1940009/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:1798929/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:1691606/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCttt                                           >  1:2150854/1‑69 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCtt                                            >  1:82820/1‑68 (MQ=255)
                   cAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTGCGCtt                                            >  1:713474/1‑68 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:2179808/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:1555617/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:1803887/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:1766257/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:10510/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:1251102/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:2689315/51‑1 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGa                                     >  1:114159/1‑68 (MQ=255)
                          ggCAAACAGATCCAACATTACCTCTCCTCATTATCAGCTTCGCGGCgcagc                                                      <  1:129881/51‑1 (MQ=255)
                           gCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:2707184/50‑1 (MQ=255)
                                 aGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCgcagc                                                      <  1:2733027/44‑1 (MQ=255)
                                  gATCCAACATTACCTCTCCTCATTTTCAACTTCGCGGCGCAGCAGTTgcgc                                              <  1:1710274/51‑1 (MQ=255)
                                             aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAAcag                  >  1:1079477/1‑66 (MQ=255)
                                             aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCg                  >  1:1912193/1‑68 (MQ=255)
                                             aCCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCg                  >  1:1665316/1‑68 (MQ=255)
                                                    ctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAgtgt          >  1:2626615/1‑69 (MQ=255)
                                                    ctcATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAgtgt          >  1:473381/1‑69 (MQ=255)
                                                      cATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGc        <  1:1441177/69‑1 (MQ=255)
                                                        ttttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGcc       >  1:2678139/1‑68 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:1139665/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:178785/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:745800/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:325783/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:1677659/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:1433136/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:918485/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:2306728/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:2245432/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGAGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:2814447/69‑1 (MQ=255)
                                                         tttCAGCTTCGCGGCGAAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     <  1:222325/69‑1 (MQ=255)
                                                          ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATc    >  1:2603524/1‑69 (MQ=255)
                                                          ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATc    >  1:1049419/1‑69 (MQ=255)
                                                          ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATc    >  1:786570/1‑69 (MQ=255)
                                                          ttCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTCCCAt     <  1:2473590/68‑1 (MQ=255)
                                                          ttCAGCTTCGCAGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCAt     >  1:1943986/1‑68 (MQ=255)
                                                            cAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATca   <  1:1679697/68‑1 (MQ=255)
                                                            cAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATca   <  1:1860944/68‑1 (MQ=255)
                                                             aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:690634/68‑1 (MQ=255)
                                                             aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:653114/68‑1 (MQ=255)
                                                             aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:3248182/68‑1 (MQ=255)
                                                             aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:2500107/68‑1 (MQ=255)
                                                             aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:2499315/68‑1 (MQ=255)
                                                             aGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATca   <  1:485847/67‑1 (MQ=255)
                                                              gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:832049/67‑1 (MQ=255)
                                                              gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:3258630/67‑1 (MQ=255)
                                                              gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:3120743/67‑1 (MQ=255)
                                                              gCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATcac  <  1:2116611/67‑1 (MQ=255)
                                                              |                                                                  
AGTGGCTCTTGCCACGGTTCAGCATCGGCAAACAGATCCAACATTACCTCTCCTCATTTTCAGCTTCGCGGCGCAGCAGTTGCGCTTTACGCGACACGCCCCAGCGGTAACCGGAAAGTGTGCCATCAC  >  minE/1436850‑1436978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A