Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,475,298 |
T→C |
31.4% |
R121R (CGT→CGC) |
nrdB → |
ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,475,298 | 0 | T | C | 31.4%
| 57.6
/ 34.3
| 70 | R121R (CGT→CGC) | nrdB | ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base T (24/24); new base C (16/6); total (40/30) |
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 |
AACTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGAT > minE/1475246‑1475355
|
aaCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:532536/69‑1 (MQ=255)
aaCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:932458/69‑1 (MQ=255)
aaCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:1506967/69‑1 (MQ=255)
aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCa < 1:1573754/68‑1 (MQ=255)
aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCAt > 1:566058/1‑69 (MQ=255)
aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCAt > 1:3033706/1‑69 (MQ=255)
aCTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCAt > 1:819645/1‑69 (MQ=255)
gAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCa > 1:3020931/1‑59 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATc > 1:3016524/1‑61 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCg > 1:1117877/1‑66 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:292721/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3334880/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:2235059/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3301467/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:807455/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:156439/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:3011036/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1499880/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:2903236/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1366073/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1349841/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTaa > 1:1229218/1‑69 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTa > 1:2617964/1‑68 (MQ=255)
aaCCTGGGTAGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTa > 1:1461213/1‑68 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGt > 1:1717461/1‑43 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:2722969/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:3314193/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:759064/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:2086212/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1998017/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:765666/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1412063/1‑69 (MQ=255)
cTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAatat > 1:1033508/1‑69 (MQ=255)
tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:611342/66‑1 (MQ=255)
tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:730469/66‑1 (MQ=255)
tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:2819949/66‑1 (MQ=255)
tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:852922/66‑1 (MQ=255)
tagAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:856927/66‑1 (MQ=255)
agAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGCTCCTATACCCATATCATCCGTAATATCGtt < 1:310449/66‑1 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTaa > 1:1698974/1‑45 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:1824576/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:2279825/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:2280113/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:431868/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:680733/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3381076/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:334982/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:252429/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3241546/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:3085005/1‑69 (MQ=255)
ctcAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCtgttgt > 1:1638149/1‑69 (MQ=255)
aCGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGAc < 1:22874/69‑1 (MQ=255)
aCGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGAc < 1:1019163/69‑1 (MQ=255)
ttcattcCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGat < 1:254314/68‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:967296/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:734348/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2257508/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:894677/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:450983/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:419739/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:3410452/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2287513/40‑1 (MQ=255)
tcattcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:2699/40‑1 (MQ=255)
attcCCGTTCCTATACTCATATCATTCGTAATATCGtt < 1:154825/38‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:195169/39‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1864840/39‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:3136446/39‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:3042000/39‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1574208/39‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:2689655/39‑1 (MQ=255)
cccGTTCCTATACTCATATCATTCGTAATATCGTTAACg < 1:1270911/39‑1 (MQ=255)
|
AACTGGAAACCTGGGTCGAAACCTGGGCGTTCTCAGAAACGATTCATTCCCGTTCCTATACTCATATCATTCGTAATATCGTTAACGATCCGTCTGTTGTGTTTGACGAT > minE/1475246‑1475355
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
