Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,486,500 |
G→A |
38.0% |
A271V (GCC→GTC) |
nuoN ← |
NADH:ubiquinone oxidoreductase, membrane subunit N |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,486,500 | 0 | G | A | 38.0%
| 47.7
/ 52.7
| 84 | A271V (GCC→GTC) | nuoN | NADH:ubiquinone oxidoreductase, membrane subunit N |
| Reads supporting (aligned to +/- strand): ref base G (30/22); new base A (12/20); total (42/42) |
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACC > minE/1486439‑1486562
|
aTACGGGCTGGACATCATGCTGACCTCGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:162840/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:3042800/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:44800/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2019826/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2118287/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1602824/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1542899/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2811818/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:3141069/69‑1 (MQ=255)
aTACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:2999054/69‑1 (MQ=255)
tACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:3374419/68‑1 (MQ=255)
tACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:73291/68‑1 (MQ=255)
tACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1816595/68‑1 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCtt > 1:1637704/1‑68 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:3350393/1‑69 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:329960/1‑69 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:1973631/1‑69 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:207274/1‑69 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:508934/1‑69 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:57734/1‑69 (MQ=255)
cAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc > 1:1374227/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCGTACCGCTTcc > 1:2545625/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:922561/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:811576/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:3229645/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:2758763/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:260481/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:2401529/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:1379639/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:660853/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTcc > 1:1701228/1‑69 (MQ=255)
aGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc < 1:2192368/68‑1 (MQ=255)
cccACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2518080/2‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGa > 1:2709375/1‑68 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:873524/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2888443/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:670446/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:776909/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2702029/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:378431/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2475505/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:1407598/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:3347999/1‑69 (MQ=255)
aCCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGAc > 1:2191737/1‑69 (MQ=255)
cACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAt < 1:1708889/69‑1 (MQ=255)
cACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACAt < 1:2258235/69‑1 (MQ=255)
cgcgCCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:535847/36‑1 (MQ=255)
gcgcCGAGGCTGCTGAACAGATAACCGGCCAGGTa < 1:1074107/35‑1 (MQ=255)
gcttctGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcgc < 1:2341033/69‑1 (MQ=255)
gctgctGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcgc < 1:3093528/69‑1 (MQ=255)
gctgctGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAgcgc < 1:3171834/69‑1 (MQ=255)
ctgctgAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCa > 1:867730/1‑69 (MQ=255)
gAACAGATAACCGGCCAGGTAAACCCCTACCGCTTc < 1:1227652/36‑1 (MQ=255)
agcAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:820866/65‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCTCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:461560/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:746417/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1764847/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:64387/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:630836/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:3028091/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1854006/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1883846/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1932857/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2089949/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2153639/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:851913/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2604918/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2887152/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1109322/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1015571/67‑1 (MQ=255)
aaCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:936327/67‑1 (MQ=255)
aCAGATAACCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:1843572/66‑1 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTg > 1:853692/1‑50 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:744013/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:1736382/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:1763058/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:1154643/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3393422/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:2299623/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3227147/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:890582/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3089751/1‑69 (MQ=255)
tAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTAcc > 1:3088813/1‑69 (MQ=255)
aCCGACCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCa < 1:2917448/59‑1 (MQ=255)
|
ATACGGGCTGGACATCAGGCTGACCACGCCGAACGCGCCGAGGCTGCTGAACAGATAACCGGCCAGGTAAACCCCTACCGCTTCCATCGACATCTCGCCGGTTTGCAGCGCAATCAGCGCTACC > minE/1486439‑1486562
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
