Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,488,482 |
G→A |
63.7% |
G182G (GGC→GGT) |
nuoM ← |
NADH:ubiquinone oxidoreductase, membrane subunit M |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,488,482 | 0 | G | A | 63.7%
| 89.8
/ 92.7
| 146 | G182G (GGC→GGT) | nuoM | NADH:ubiquinone oxidoreductase, membrane subunit M |
| Reads supporting (aligned to +/- strand): ref base G (31/22); new base A (48/45); total (79/67) |
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 |
Only 100 of 162 total aligned reads displayed.
GTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTT > minE/1488437‑1488549
|
gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1021568/1‑68 (MQ=255)
gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:1831518/1‑68 (MQ=255)
gTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATga > 1:414919/1‑68 (MQ=255)
aGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaag < 1:2225037/68‑1 (MQ=255)
gTGAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACT‑‑CCTGGGTGTAAATGAAGAAc < 1:405860/68‑1 (MQ=255)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:13136/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1894943/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:592725/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:147032/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1410425/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:1409243/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2564492/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2858643/68‑1 (MQ=39)
gAACGAAAACCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAAc < 1:2981725/68‑1 (MQ=39)
aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt > 1:738888/1‑69 (MQ=255)
aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt > 1:2726750/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTgg > 1:463226/1‑47 (MQ=37)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2273504/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:3013666/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2304703/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:2379688/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:381033/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1851817/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1790918/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:6562/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1084670/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1746668/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACCAGACCACTCCCCTGGGTGTAAATGAAGAACTTGGTTg > 1:1562615/1‑69 (MQ=255)
aaaCCAGCGCCAGGATGGCGATCAACATCACAAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg > 1:730549/1‑69 (MQ=255)
ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:1287423/1‑69 (MQ=255)
ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:1000563/1‑69 (MQ=255)
ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccg > 1:2835546/1‑69 (MQ=255)
ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgcc > 1:936533/1‑68 (MQ=255)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3389345/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2801593/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1126520/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3378263/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1186067/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2479723/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:3031068/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2936488/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:764556/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:2255036/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1631176/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1506893/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1601037/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:1914240/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:682596/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:561947/55‑1 (MQ=38)
gATGGCGATCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGtt < 1:215467/55‑1 (MQ=38)
gATGGCGAGCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGAtt < 1:2309748/55‑1 (MQ=37)
tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:2749390/1‑69 (MQ=255)
tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcg > 1:1972794/1‑69 (MQ=255)
tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGCGATAcgcg > 1:1612076/1‑69 (MQ=255)
gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGa < 1:129519/61‑1 (MQ=255)
tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:541461/69‑1 (MQ=255)
tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:1940732/69‑1 (MQ=255)
tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:2542963/69‑1 (MQ=255)
tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc < 1:806771/69‑1 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGGCGTGATACGCGt > 1:867784/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:356774/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:496623/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:3249244/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:266813/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:120453/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2102240/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1960801/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2200817/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1230529/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2342133/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2601122/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2879555/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:2898114/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1247674/1‑64 (MQ=255)
tCAACATCACCAGACCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt > 1:1704808/1‑64 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:2835625/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:3400974/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:912336/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc < 1:3263674/69‑1 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:845129/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:820578/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:2195086/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc > 1:937421/1‑69 (MQ=255)
cAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGATTTAcc > 1:1263246/1‑69 (MQ=255)
cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:2920091/68‑1 (MQ=255)
cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:1107659/68‑1 (MQ=255)
cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:655450/68‑1 (MQ=255)
cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:1562238/68‑1 (MQ=255)
cAGGCCACTCGCCTGGGTGTAAATGAAGAACTTAGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg < 1:966231/68‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2127005/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:1443244/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:6074/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:439724/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:439464/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:225216/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:2697603/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3428985/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3171229/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3096052/69‑1 (MQ=255)
ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt < 1:3069322/69‑1 (MQ=255)
|
GTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTT > minE/1488437‑1488549
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
