Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,505,836 |
A→G |
22.6% |
G501G (GGT→GGC) |
yfbS ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,505,836 | 0 | A | G | 22.6%
| 53.8
/ 10.8
| 44 | G501G (GGT→GGC) | yfbS | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (23/11); new base G (5/5); total (28/16) |
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-02 |
AAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGT > minE/1505773‑1505890
|
aaaTAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGcc < 1:2917957/69‑1 (MQ=255)
aaaTAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGcc < 1:1738058/69‑1 (MQ=255)
aaTAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGcc < 1:1571196/68‑1 (MQ=255)
tAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAAt > 1:2951204/1‑69 (MQ=255)
aGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATg < 1:1335371/69‑1 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCa > 1:2069350/1‑65 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:1955936/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:2642875/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:2521224/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:2672126/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:2240270/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:323409/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:2050538/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:196487/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:421036/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:1815605/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:504616/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:648902/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:713820/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:781215/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAg > 1:1030110/1‑69 (MQ=255)
aaCCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATAAg > 1:880651/1‑69 (MQ=255)
acaagacaaACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTc < 1:2837577/68‑1 (MQ=255)
gacaaACAAACAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGc < 1:2158003/69‑1 (MQ=255)
gacaaACAAACAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGc < 1:1551177/69‑1 (MQ=255)
gacaaACAAACAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGc < 1:1829722/69‑1 (MQ=255)
acaaacaaacaaCCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGc < 1:468041/68‑1 (MQ=255)
caaacaaaCAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGCCa > 1:2798308/1‑69 (MQ=255)
caaacaaaCAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGCCa > 1:695049/1‑69 (MQ=255)
caaacaaaCAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGCCa > 1:2944992/1‑69 (MQ=255)
caaacaaaCAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGCCa > 1:2982651/1‑69 (MQ=255)
caaacaaaCAACCCAGCATCATATGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGCCa > 1:2139193/1‑69 (MQ=255)
caaacaCCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAgcgc < 1:3138759/69‑1 (MQ=255)
atatGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTc > 1:1268125/1‑69 (MQ=255)
atatGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTc > 1:2547391/1‑69 (MQ=255)
atatGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTc > 1:730763/1‑69 (MQ=255)
atatGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTc > 1:2325473/1‑69 (MQ=255)
tatGTGGCCCGTAACCGCCGCCAATGTCCATCAGCCCTTTCACCGc < 1:3180439/46‑1 (MQ=255)
tgtgGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGt < 1:1292476/69‑1 (MQ=255)
tgtgGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGt < 1:2921975/69‑1 (MQ=255)
tgtgGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGt < 1:1285552/69‑1 (MQ=255)
tgtgGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGt > 1:858191/1‑69 (MQ=255)
tgtgGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGt < 1:2466504/69‑1 (MQ=255)
tgtgGCCCGTAACCACCGCCAATGTCCATCAGACCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGt < 1:1288718/69‑1 (MQ=255)
|
AAATAGCCCAATAACCGCCGACAAGACAAACAAACACCCCAGCATCATATGTGGCCCGTAACCACCGCCAATGTCCATCAGCCCTTTCACCGCCAGCGCGACACCTCCCGTTTTCTGT > minE/1505773‑1505890
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
