Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,506,604 |
C→T |
62.1% |
R245R (CGG→CGA) |
yfbS ← |
predicted transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,506,604 | 0 | C | T | 62.1%
| 56.4
/ 71.3
| 108 | R245R (CGG→CGA) | yfbS | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (24/17); new base T (45/22); total (69/39) |
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01 |
Only 100 of 108 total aligned reads displayed.
AGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATA > minE/1506546‑1506669
|
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:2450911/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:3325288/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:1944691/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:2846184/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:1562906/69‑1 (MQ=255)
aGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:1353016/69‑1 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACTTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:2404824/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTg > 1:94951/1‑63 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGCCCAAt > 1:1492457/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCaa > 1:1265655/1‑68 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:22446/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:3224354/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:3059620/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:201513/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:1996005/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:246128/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:345296/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:411442/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:1902827/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:891384/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:3018104/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:3355321/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:624061/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:1664365/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:2589399/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:2403023/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:818378/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:687173/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:757971/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:770109/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:814195/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:895347/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:2546606/1‑69 (MQ=255)
gCGTTCAACACCGATGACGTTAGCACCATAACGCTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:2421059/1‑69 (MQ=255)
gCGTTCAACACAGATGACGTTAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAAt > 1:1746644/1‑69 (MQ=255)
acCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGa < 1:134659/69‑1 (MQ=255)
gATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc < 1:1590121/69‑1 (MQ=255)
aTGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATcc < 1:626901/68‑1 (MQ=255)
gACGTTTGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCgg > 1:441444/1‑68 (MQ=255)
gACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCaa < 1:1243018/53‑1 (MQ=255)
gACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCggg > 1:2054223/1‑69 (MQ=255)
gACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCggg > 1:597353/1‑69 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:2928920/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:3046915/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:2924991/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:2425349/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:236819/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:2084769/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:2052085/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:3380156/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:526215/69‑1 (MQ=255)
tAGCACCATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGaa < 1:1138439/69‑1 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCg > 1:86768/1‑52 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:3336402/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:3269526/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:3372213/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:314851/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:2678373/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:1022097/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:277893/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:1228404/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:1377213/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:1482271/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:1000889/1‑66 (MQ=255)
ccATAACGTTCACGTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATa > 1:2946549/1‑66 (MQ=255)
cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:428290/69‑1 (MQ=255)
cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:10652/69‑1 (MQ=255)
cATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:458030/69‑1 (MQ=255)
aTAACGCTCACGTAATTTGAGATCATCCAGCCTTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:741905/68‑1 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGAc > 1:1483738/1‑68 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg > 1:2418544/1‑69 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg > 1:2832073/1‑69 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg > 1:1218260/1‑69 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg > 1:801023/1‑69 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg > 1:1701211/1‑69 (MQ=255)
aCGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACg > 1:450307/1‑69 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGTGGCGAATAGCCAGACGTCGCGCa < 1:3386470/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:2125490/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:103334/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:1615824/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:586569/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:1774913/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:1954198/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:394952/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:379499/68‑1 (MQ=255)
gTAATTTGAGATCGTCCAGTCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCa < 1:3396368/68‑1 (MQ=255)
tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg > 1:1875655/1‑64 (MQ=255)
tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg > 1:2072737/1‑64 (MQ=255)
tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACg > 1:2718964/1‑64 (MQ=255)
tGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCg > 1:2593758/1‑68 (MQ=255)
atcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCg < 1:2877431/69‑1 (MQ=255)
atcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCg < 1:823289/69‑1 (MQ=255)
atcCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCg < 1:3204901/69‑1 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGata > 1:1976367/1‑69 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGata > 1:1984726/1‑69 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGata > 1:1671586/1‑69 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGata > 1:2648942/1‑69 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGat > 1:3189581/1‑68 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGa > 1:1361532/1‑67 (MQ=255)
cAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCAGTCAGTCGa > 1:33868/1‑67 (MQ=255)
|
AGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTCGCGCACGCCCGGTCAGTCGATA > minE/1506546‑1506669
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
