Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,514,054	G→A	34.5%	L479L (CTC→CTT)	purF ←	amidophosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,514,054	0	G	A	34.5%	100.9 / 74.2	130	L479L (CTC→CTT)	purF	amidophosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base G (20/65); new base A (28/17); total (48/82)
Fisher's exact test for biased strand distribution p-value = 2.12e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

TTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCAAA  >  minE/1513990‑1514122

tttCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa                                                                  >  1:3170/1‑69 (MQ=255)

tttCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa                                                                  >  1:3197977/1‑69 (MQ=255)

tttCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGa                                                                  >  1:1395409/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCg                                                             >  1:1830335/1‑68 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCg                                                             >  1:3207894/1‑68 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:3319767/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:617607/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:54423/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:1925691/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:2057438/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:3340272/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:2184654/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:1028691/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:2269804/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:2363571/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:2987142/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:2907528/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:1189688/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:1462073/1‑69 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:900879/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:2321138/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:1250265/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:540564/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:268146/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:1505657/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:1577571/68‑1 (MQ=255)

      gaTTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:839451/68‑1 (MQ=255)

      gaTTTTCCACTTCATTCTGACGTTGCACTGCTTTGGCGTCGTCATTACGTAAAGTATCAAGGAAATCGa                                                            >  1:3087875/1‑69 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:1982426/68‑1 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:1519833/68‑1 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:1188625/68‑1 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:1013989/68‑1 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:2090590/68‑1 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:1336135/68‑1 (MQ=255)

                    ttCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATc                                               <  1:573117/68‑1 (MQ=255)

                         aCGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCg                                                             <  1:1574104/49‑1 (MQ=255)

                         aCGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATc                                         >  1:1066177/1‑69 (MQ=255)

                                aCTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:916036/68‑1 (MQ=255)

                                aCTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:2150684/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGTTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1886549/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:2482994/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1098826/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1146916/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1327528/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:2384956/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:2580171/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:293505/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:3161153/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:333359/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             >  1:125876/1‑68 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:2093022/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:3353418/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1942951/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1933605/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1885526/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:850862/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1424343/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:171894/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:1576849/68‑1 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAg                            >  1:494987/1‑69 (MQ=255)

                                      ttGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAg                            >  1:1439583/1‑69 (MQ=255)

                                       tGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             <  1:2387347/67‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:2859624/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:944671/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:929222/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:1571749/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:724819/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:1832379/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:2254192/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:2479629/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:2904902/68‑1 (MQ=255)

                                          cgtcgtCATTACGTAAAGTATCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:2838237/68‑1 (MQ=255)

                                              atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGccat                     >  1:652257/1‑66 (MQ=255)

                                              atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCg                      >  1:473672/1‑67 (MQ=255)

                                              atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGt                     >  1:2073931/1‑68 (MQ=255)

                                              atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGt                     >  1:835625/1‑68 (MQ=255)

                                              atcatTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGt                     >  1:1402661/1‑68 (MQ=255)

                                                     cgtaaagtaTCAAGGAAATCGAGGTAGCCCTGATCAACGTCTTTGGTGACGTAGACg                         <  1:3275568/57‑1 (MQ=255)

                                                       taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg            >  1:2643284/1‑68 (MQ=255)

                                                       taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg            >  1:1948669/1‑68 (MQ=255)

                                                       taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg            >  1:297911/1‑68 (MQ=255)

                                                       taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg            >  1:1664484/1‑68 (MQ=255)

                                                       taacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCg            >  1:2726290/1‑68 (MQ=255)

                                                        aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:2239454/44‑1 (MQ=255)

                                                        aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:2820401/44‑1 (MQ=255)

                                                        aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:44434/44‑1 (MQ=255)

                                                        aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:1830472/44‑1 (MQ=255)

                                                        aacgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAg          <  1:618897/69‑1 (MQ=255)

                                                        aacgtaTCGAGGAAATCGAGGTAGACCTGATCAACATCTTTGGt                                   <  1:1359174/44‑1 (MQ=255)

                                                         acgtaTCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGt                                   <  1:1698700/43‑1 (MQ=255)

                                                               cGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaa   >  1:2387473/1‑69 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             >  1:2842164/1‑42 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             >  1:2567167/1‑42 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTa                             >  1:3150878/1‑42 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa  >  1:369066/1‑69 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa  >  1:2089473/1‑69 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa  >  1:2808918/1‑69 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa  >  1:3242281/1‑69 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa  >  1:3087165/1‑69 (MQ=255)

                                                                gAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCaaa  >  1:1126486/1‑69 (MQ=255)

TTTCGAGATTTTCCACTTCGTTCTGACGTTGCACTGCTTTGGCGTCATCATTACGTAACGTATCGAGGAAATCGAGGTAGCCCTGATCAACATCTTTGGTGACGTAGACGCCGTTGAACACCGAGCATTCAAA  >  minE/1513990‑1514122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A