Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,550,810 T→C 38.3% G86G (GGT→GGC)  yfdC → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,550,8100TC38.3% 41.5 / 56.5 86G86G (GGT→GGC) yfdCpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (8/45);  new base C (15/18);  total (23/63)
Fisher's exact test for biased strand distribution p-value = 2.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 101 total aligned reads displayed.

GCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGCCC  >  minE/1550745‑1550877
                                                                 |                                                                   
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGgtgt                                                                   >  1:1525518/1‑68 (MQ=255)
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGtgtg                                                                  >  1:2163412/1‑69 (MQ=255)
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGtgtg                                                                  >  1:939912/1‑69 (MQ=255)
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGtgtg                                                                  >  1:28000/1‑69 (MQ=255)
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGtgtg                                                                  >  1:1386294/1‑69 (MQ=255)
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGtgtg                                                                  >  1:2501647/1‑69 (MQ=255)
gcggcgGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGtgtg                                                                  >  1:1330927/1‑69 (MQ=255)
            tCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGcttc                                                      >  1:3244082/1‑69 (MQ=255)
            tCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGcttc                                                      >  1:2679552/1‑69 (MQ=255)
                     gcTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:1584001/69‑1 (MQ=255)
                     gcTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:660928/69‑1 (MQ=255)
                     gcTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:1957808/69‑1 (MQ=255)
                     gcTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:1291960/69‑1 (MQ=255)
                     gcTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:1218015/69‑1 (MQ=255)
                     gcTTCGCTACTGGCTAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:695285/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:604269/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:593811/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:2810388/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:2150240/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:2766676/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:147723/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:1582361/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:2473108/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:676892/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:1307864/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:891579/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:1199770/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:2567414/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:1115389/68‑1 (MQ=255)
                     gcTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTCCTGga                                              <  1:2379646/68‑1 (MQ=255)
                      cTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:789191/68‑1 (MQ=255)
                      cTTCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:616596/67‑1 (MQ=255)
                        tCGCTACTGGCAAAAGGGATATTTCATGTCGAACTGGAAGGCGTGCCGGGCAGCTTCTTGCTGga                                              <  1:5364/65‑1 (MQ=255)
                             aCTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg                                     <  1:3363098/69‑1 (MQ=255)
                             aCTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg                                     <  1:372422/69‑1 (MQ=255)
                             aCTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg                                     <  1:107377/69‑1 (MQ=255)
                             aCTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg                                     <  1:1559455/69‑1 (MQ=255)
                             aCTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg                                     <  1:1727716/69‑1 (MQ=255)
                             aCTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCg                                      >  1:2563690/1‑68 (MQ=255)
                               tGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGt                                    <  1:2777937/68‑1 (MQ=255)
                               tGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGt                                    <  1:59293/68‑1 (MQ=255)
                               tGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGt                                    <  1:2987560/68‑1 (MQ=255)
                               tGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGt                                    <  1:226229/68‑1 (MQ=255)
                                ggCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTta                                  <  1:869359/69‑1 (MQ=255)
                                ggCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTta                                  <  1:1139143/69‑1 (MQ=255)
                                ggCAAAAGGGATATTTCAAGTAGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:1598831/58‑1 (MQ=255)
                                   aaaaGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTtat                                 <  1:2771283/67‑1 (MQ=255)
                                   aaaaGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTtat                                 <  1:791840/67‑1 (MQ=255)
                                         gATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTgg                         >  1:2595880/1‑69 (MQ=255)
                                         gATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTgg                         >  1:2235355/1‑69 (MQ=255)
                                         gATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTgg                         >  1:2034082/1‑69 (MQ=255)
                                         gATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTgg                         >  1:2446939/1‑69 (MQ=255)
                                            atTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGttt                      <  1:2537272/69‑1 (MQ=255)
                                               tCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGgag                                             <  1:2058657/43‑1 (MQ=255)
                                                cAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTttatt                  >  1:504848/1‑69 (MQ=255)
                                                 aaGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTttatt                  <  1:1349135/68‑1 (MQ=255)
                                                 aaGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTttatt                  <  1:2726598/68‑1 (MQ=255)
                                                 aaGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTttatt                  <  1:2821022/68‑1 (MQ=255)
                                                 aaGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTttatt                  <  1:198742/68‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTTCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:1634701/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTTGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:2124906/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCac          <  1:2689953/69‑2 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:640411/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:1430084/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:726718/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:777207/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:2091445/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:1309637/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:1205620/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:961416/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:387144/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:445787/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:2886648/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:2749630/69‑1 (MQ=255)
                                                        aCTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:2325078/69‑1 (MQ=255)
                                                         cTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCAt          <  1:2229666/68‑1 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:249696/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:345640/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:2635119/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:934690/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:1285474/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:2643958/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:84827/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:1385748/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:727643/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:2325980/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:1906152/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:2931498/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:560643/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAtt      >  1:3123191/1‑68 (MQ=255)
                                                            gAAGGCGTGCCGGGCAGCGTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATAAt       >  1:3074153/1‑68 (MQ=255)
                                                             aaGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCgg                                     <  1:470368/37‑1 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTc            >  1:2308247/1‑58 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:2779465/1‑68 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:1386303/1‑68 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:2333404/1‑68 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:2495936/1‑68 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:2266139/1‑68 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:507574/1‑68 (MQ=255)
                                                                 tgtgCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGccc  >  1:914905/1‑68 (MQ=255)
                                                                 |                                                                   
GCGGCGGGTCTGTCGATGGGCGCTTCGTTACTGGCAAAAGGGATATTTCAAGTCGAACTGGAAGGTGTGCCGGGCAGCTTCTTGCTGGAGAATCTCGGTTATACCTTTGGTTTTATTATCGTCATTATGGCCC  >  minE/1550745‑1550877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A