Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,571,749 A→G 31.3% V75V (GTA→GTG)  yfeC → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,571,7490AG31.3% 90.9 / 53.0 105V75V (GTA→GTG) yfeCpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (27/45);  new base G (2/31);  total (29/76)
Fisher's exact test for biased strand distribution p-value = 7.55e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 109 total aligned reads displayed.

GCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTT  >  minE/1571687‑1571812
                                                              |                                                               
gCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg                                                           >  1:692673/1‑69 (MQ=255)
gCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg                                                           >  1:2697817/1‑69 (MQ=255)
 cTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGTGCCGCTTGAAGTATTGCTg                                                           >  1:907239/1‑68 (MQ=255)
 cTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg                                                           >  1:2525965/1‑68 (MQ=255)
 cTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTg                                                           >  1:811394/1‑68 (MQ=255)
     aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGac                                                      >  1:2590210/1‑69 (MQ=255)
     aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       >  1:1606966/1‑68 (MQ=255)
     aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       >  1:3348284/1‑68 (MQ=255)
     aCGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTCCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGac                                                      >  1:2065091/1‑69 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTTATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:796450/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:2110403/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:2468236/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:2390352/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:2046207/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:529437/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:2469950/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:237948/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:454915/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:383985/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:1558251/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:3033358/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:2602498/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:3287174/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:1018115/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:1569058/67‑1 (MQ=255)
      cGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:772311/67‑1 (MQ=255)
       gTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGa                                                       <  1:1067759/66‑1 (MQ=255)
        tCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACt                                                   <  1:1059014/69‑1 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAGGTATTGCTGGTGACACTg                                                  >  1:1766079/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:1814462/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:1463611/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:1780275/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:2519888/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:2074288/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:3384073/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:3395482/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:1729483/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:748755/1‑68 (MQ=255)
          cAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGGGATGCGCCGCTTGAAGTATTGCTGGTGACACTg                                                  >  1:2658302/1‑68 (MQ=255)
             aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa                                              <  1:268276/69‑1 (MQ=255)
             aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa                                              <  1:2134983/69‑1 (MQ=255)
             aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa                                              <  1:2316579/69‑1 (MQ=255)
             aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa                                              <  1:1066165/69‑1 (MQ=255)
             aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa                                              <  1:1974525/69‑1 (MQ=255)
             aaGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGa                                              <  1:1356098/69‑1 (MQ=255)
                   aGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATT‑CTGGTGACACTGGCGAAAGAGAt                                       <  1:2614714/69‑1 (MQ=255)
                     ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt                                       <  1:316423/68‑1 (MQ=255)
                     ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt                                       <  1:298991/68‑1 (MQ=255)
                     ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt                                       <  1:2449516/68‑1 (MQ=255)
                     ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt                                       <  1:933882/68‑1 (MQ=255)
                     ggAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGAt                                       <  1:1511674/68‑1 (MQ=255)
                          aGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCgt                                              <  1:1270568/56‑2 (MQ=255)
                                      ttCTGGTGATGCGCCGCTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACaaa                      >  1:435523/1‑68 (MQ=255)
                                      ttCTGGTGATGCGCCGCTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACaaa                      >  1:722693/1‑68 (MQ=255)
                                      ttCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACaaaa                     <  1:2223567/69‑1 (MQ=255)
                                          ggTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACa                  <  1:2910535/68‑1 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:1517336/1‑69 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:1775724/1‑69 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             <  1:1218522/69‑1 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:2458571/1‑69 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:3311094/1‑69 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             <  1:1072916/69‑1 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             <  1:2205278/69‑1 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             <  1:3141731/69‑1 (MQ=255)
                                              aTGCGCCGCTTGAAGTATTGCTGGGGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             <  1:254806/69‑1 (MQ=255)
                                               tGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             <  1:3145465/68‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:976351/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:962780/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:554928/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:2218514/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:1027111/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:1161262/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:1504543/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:1976112/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:2303244/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:2342770/69‑1 (MQ=255)
                                                    cgcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:2959134/69‑1 (MQ=255)
                                                    cgcTTGAAGTATTGCTGGTGACACTGGCGAAATAGAt                                       <  1:3421112/37‑1 (MQ=255)
                                                     gcTTGAAGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCt       <  1:3144825/68‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGATATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:2259651/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:560402/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:3116194/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:1096764/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:1107547/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:1122568/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:3083636/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:1184648/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:3258169/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:765076/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:1622841/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:332428/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:556323/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:532489/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:2994725/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:2447181/67‑1 (MQ=255)
                                                           aGTGTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCtt  <  1:2888264/67‑1 (MQ=255)
                                                              aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:1623199/1‑53 (MQ=255)
                                                              aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:3033073/1‑53 (MQ=255)
                                                              aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:2137276/1‑53 (MQ=255)
                                                              aTTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTa             >  1:3319931/1‑53 (MQ=255)
                                                              |                                                               
GCTGAACGTCCAGAAGGTCAGGGAGAAGCGCCTGCCCTTTCTGGTGATGCGCCGCTTGAAGTATTGCTGGTGACACTGGCGAAAGAGATGACGCCAGTTGAACAAAAACAGTTTACATCCTTGCTT  >  minE/1571687‑1571812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A