Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,612,073 |
C→T |
44.1% |
Q360Q (CAG→CAA) |
eutA ← |
reactivating factor for ethanolamine ammonia lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,612,073 | 0 | C | T | 44.1%
| 16.6
/ 53.0
| 71 | Q360Q (CAG→CAA) | eutA | reactivating factor for ethanolamine ammonia lyase |
| Reads supporting (aligned to +/- strand): ref base C (9/30); new base T (10/21); total (19/52) |
| Fisher's exact test for biased strand distribution p-value = 4.28e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
GTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCA > minE/1612007‑1612137
|
gTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTg < 1:666643/69‑1 (MQ=255)
tAACGCATAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2404313/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:9830/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2579801/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:1307234/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:1856232/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:2143613/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:163095/69‑1 (MQ=255)
tAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGa < 1:413125/69‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3049725/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:257595/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2179688/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2968649/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2809380/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2766479/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2598080/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2425130/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2587846/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3083994/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:3243509/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1621681/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1520207/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1462152/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:446669/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:472156/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:474538/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:515934/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:1265533/67‑1 (MQ=255)
aCGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:840527/67‑1 (MQ=255)
aCGCACAGGCAGCGAGCCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGa < 1:2279345/67‑1 (MQ=255)
gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:3387491/69‑1 (MQ=255)
gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2975654/69‑1 (MQ=255)
gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2817081/69‑1 (MQ=255)
gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:985722/69‑1 (MQ=255)
gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2489156/69‑1 (MQ=255)
gCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2204798/69‑1 (MQ=255)
cGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2284128/68‑1 (MQ=255)
aGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCTTGTTGc < 1:4499/68‑1 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGcc > 1:2777597/1‑68 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1166626/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:610392/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1370616/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:202669/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:695394/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:219918/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:2949640/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:886534/1‑69 (MQ=255)
ggCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGGCAAAGATCCAGTTGAATCAGCGCCTGTTGCCa > 1:1389468/1‑69 (MQ=255)
aGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGccccc > 1:3151785/1‑69 (MQ=255)
aGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGcccc > 1:1386367/1‑68 (MQ=255)
gCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGtt < 1:2155431/51‑1 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCCGCGCCTGTTGCCAGGCCCCCACCAGAt > 1:2575353/1‑56 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:491111/1‑69 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:40910/1‑69 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:3398330/1‑69 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:2669320/1‑69 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:1098557/1‑69 (MQ=255)
aGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAAt > 1:2494811/1‑69 (MQ=255)
ttGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCggg < 1:2420082/69‑1 (MQ=255)
ttGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCggg < 1:1389875/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:607864/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1043488/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1602166/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1821658/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1909804/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2207739/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2635437/69‑1 (MQ=255)
ccAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:244984/69‑1 (MQ=255)
ccAGATGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2714844/69‑1 (MQ=255)
cAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:1449531/68‑1 (MQ=255)
cAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCa < 1:2128642/68‑1 (MQ=255)
|
GTAACGCACAGGCAGCGAGGCGGGAAGCGCCAGCACGTACGCGTCAGTTTTGGGATCAAGATCCAGCTGAATCAGCGCCTGTTGCCAGGCCCCCACCAGATCCGTTTCATCAATCGGGATCGCCACCGGCA > minE/1612007‑1612137
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
