Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,614,100 |
C→T |
32.6% |
S92S (TCG→TCA) |
eutH ← |
predicted inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,614,100 | 0 | C | T | 32.6%
| 89.8
/ 53.8
| 104 | S92S (TCG→TCA) | eutH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (41/29); new base T (11/23); total (52/52) |
| Fisher's exact test for biased strand distribution p-value = 2.08e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 |
Only 100 of 104 total aligned reads displayed.
AGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAG > minE/1614041‑1614162
|
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:906532/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:214796/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:831097/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:737851/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:2153892/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:2398029/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:2779438/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGttt > 1:3376136/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGtt > 1:1810113/1‑68 (MQ=255)
aGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGtt > 1:47813/1‑68 (MQ=255)
tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:3356604/1‑68 (MQ=255)
tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:2337784/1‑68 (MQ=255)
tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:2820735/1‑68 (MQ=255)
tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:1475295/1‑68 (MQ=255)
tGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg > 1:3031460/1‑68 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2846322/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2829075/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2982459/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:3093649/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2656710/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:1289176/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:1168240/66‑1 (MQ=255)
gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:2127355/66‑1 (MQ=255)
cGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATTGACGGGTTTGCGCCg < 1:707125/65‑1 (MQ=255)
gAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCa < 1:2849535/68‑1 (MQ=255)
gAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCa < 1:480332/68‑1 (MQ=255)
gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAttt > 1:257686/1‑68 (MQ=255)
gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAttt > 1:1278327/1‑68 (MQ=255)
gaagaaGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAttt > 1:1786662/1‑68 (MQ=255)
gccgccCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAAc > 1:2061179/1‑69 (MQ=255)
gccgccCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAAc > 1:2632417/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2778495/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2274850/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:163946/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:765415/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2443083/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1584872/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:273804/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:69551/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2666445/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:2830508/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1404567/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1302149/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:3378654/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1253012/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:846286/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1889372/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:1189587/1‑69 (MQ=255)
tCGCACGCCAGCAGTGTTCAGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt > 1:471121/1‑69 (MQ=255)
gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:2250776/1‑69 (MQ=255)
gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:609301/1‑69 (MQ=255)
gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:3033940/1‑69 (MQ=255)
gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:1715359/1‑69 (MQ=255)
gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:214243/1‑69 (MQ=255)
gCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAc > 1:277978/1‑69 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2253721/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:3242176/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:1328915/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2956388/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:1523140/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:810761/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2189482/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:2198448/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:652160/61‑1 (MQ=255)
aCGCCAGCAGTGTTCCGGCGAACATCGATGGGGTAGCGCCGAGCATTTCGTAAACCGGaat < 1:544331/61‑1 (MQ=255)
cGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaat < 1:1933720/60‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:3151585/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2257211/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:449970/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:734549/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2599129/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2050845/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:642992/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2077571/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:146817/69‑1 (MQ=255)
cagcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:1528004/69‑1 (MQ=255)
agcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:2496352/68‑1 (MQ=255)
agcagTGTTCCGGCGAACATTGACGGGTTTGCGCCGAGCATTTCGTAAACCGGGATAATCACCGGccc < 1:510590/68‑1 (MQ=255)
ttCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCgag > 1:1733033/1‑64 (MQ=255)
ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACTGGAATAATTACCGGCCCGAGAACGTGa > 1:1296209/1‑69 (MQ=255)
ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:608274/1‑69 (MQ=255)
ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:1805040/1‑69 (MQ=255)
ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:2811604/1‑69 (MQ=255)
ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:1398762/1‑69 (MQ=255)
ccGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGa > 1:3167011/1‑69 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:3411360/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1163801/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1623227/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1194167/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:3204710/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:564640/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1289432/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:527453/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1258493/69‑1 (MQ=255)
gCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACCTGAGcc < 1:2053506/69‑1 (MQ=255)
gAAGATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1808836/67‑1 (MQ=255)
gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAg < 1:2760370/69‑1 (MQ=255)
gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAg < 1:114486/69‑1 (MQ=255)
gAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAg < 1:2248000/69‑1 (MQ=255)
tCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc < 1:1218089/62‑1 (MQ=255)
|
AGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAG > minE/1614041‑1614162
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
