Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,616,715 |
G→C |
100% |
T435T (ACC→ACG) |
eutE ← |
predicted aldehyde dehydrogenase, ethanolamine utilization protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,616,715 | 0 | G | C | 100.0%
| 17.1
/ NA
| 9 | T435T (ACC→ACG) | eutE | predicted aldehyde dehydrogenase, ethanolamine utilization protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (0/9); total (0/9) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCA > minE/1616669‑1616737
|
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCATCCTTCCCCGccca < 1:2235035/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:1712672/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:2053511/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:2140654/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:3045666/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:633417/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:705105/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:812087/67‑1 (MQ=255)
tccgcgcgCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATCGTGGTCCAGCCTTCCCCGccca < 1:908446/67‑1 (MQ=255)
|
TACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCA > minE/1616669‑1616737
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
