Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,633,534 |
T→C |
45.6% |
G512G (GGT→GGC) |
narQ → |
sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,633,534 | 0 | T | C | 45.6%
| 11.0
/ 75.7
| 90 | G512G (GGT→GGC) | narQ | sensory histidine kinase in two‑component regulatory system with NarP (NarL) |
| Reads supporting (aligned to +/- strand): ref base T (33/16); new base C (23/18); total (56/34) |
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGC > minE/1633479‑1633583
|
gCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAAcc > 1:3435933/1‑67 (MQ=255)
gCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAAcc > 1:3323721/1‑67 (MQ=255)
cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:326766/69‑1 (MQ=255)
cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:2516715/69‑1 (MQ=255)
cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:1438784/69‑1 (MQ=255)
cACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:2520848/69‑1 (MQ=255)
aCCGCGCCGTACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:3241011/1‑69 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGaaa < 1:556631/61‑2 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2568942/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2644226/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2974562/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:3033088/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:353217/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:37100/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2080436/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2058506/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2023913/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:2001778/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:1963468/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:1013953/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:752040/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:892916/61‑1 (MQ=255)
aCCGCGCCGGATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:1462525/61‑1 (MQ=255)
aCCGCGCCGGATGACAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:166545/61‑1 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2016668/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2649093/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:1666209/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:1650083/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:3126119/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:944571/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:3093622/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2487172/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGaa > 1:2913046/1‑69 (MQ=255)
aCCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGa < 1:97783/68‑1 (MQ=255)
aCCGCGCC‑GATGGCAATCACACAGTCTATATTCGCGACAACGGGATTGGCATCGGTGAAc < 1:907889/60‑1 (MQ=255)
tcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:2831842/2‑68 (MQ=255)
gcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:768402/1‑68 (MQ=255)
gcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:3408336/1‑68 (MQ=255)
gcgcCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc > 1:270501/1‑68 (MQ=255)
cGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAg < 1:348808/69‑1 (MQ=255)
acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:2768475/1‑68 (MQ=255)
acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:1406361/1‑68 (MQ=255)
acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:3014736/1‑68 (MQ=255)
acacGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGt > 1:645201/1‑68 (MQ=255)
ttATATTCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:441647/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGaaa > 1:2301360/1‑40 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:1417266/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:3433130/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:2274134/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:1157487/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:742873/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:68106/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:3150190/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:3139942/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:1816704/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAg > 1:195205/1‑41 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1063339/69‑1 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:252171/69‑1 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1837728/69‑1 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1787130/69‑1 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1306956/69‑1 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:1088279/69‑1 (MQ=255)
ttATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat < 1:3069985/69‑1 (MQ=255)
tataTCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTg < 1:914314/63‑1 (MQ=255)
tataTCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTg < 1:239041/63‑1 (MQ=255)
tCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATgc > 1:1239349/1‑69 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:946550/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1173032/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:90068/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:994286/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:159683/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:767058/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1621864/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3095366/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1808971/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:1857189/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:492753/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:404004/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2165866/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2253647/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2347877/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:246209/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:335600/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:2985394/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3006792/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3244194/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3016622/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGTCTGAata > 1:3034686/1‑53 (MQ=255)
cGGGATTGGCATCGGTGAACCGAAAGAACCCGAAGGCCATTATGGGCTGAat > 1:1904761/1‑52 (MQ=255)
gATTGGTATAGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTg < 1:3358390/46‑1 (MQ=255)
|
GCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGC > minE/1633479‑1633583
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
