Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,699,077 |
T→C |
38.9% |
S319S (AGT→AGC) |
csiE → |
stationary phase inducible protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,699,077 | 0 | T | C | 38.9%
| 25.7
/ 37.3
| 59 | S319S (AGT→AGC) | csiE | stationary phase inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (26/10); new base C (11/12); total (37/22) |
| Fisher's exact test for biased strand distribution p-value = 9.68e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
CACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTGCATGAAAAACAGAT > minE/1699011‑1699142
|
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1188665/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1109687/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2843942/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2359789/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2199800/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1351751/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:3111178/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2052624/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:3270521/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1694819/68‑1 (MQ=255)
caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:490956/68‑1 (MQ=255)
ccaccCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtggtc > 1:2509248/1‑69 (MQ=255)
accCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtggtctg > 1:298753/1‑69 (MQ=255)
accCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtggtctg > 1:1932447/1‑69 (MQ=255)
ggcgCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGt < 1:497981/69‑1 (MQ=255)
gcgcTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTg > 1:358808/1‑69 (MQ=255)
gcgcTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTg > 1:3358450/1‑69 (MQ=255)
ggATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGt < 1:516141/69‑1 (MQ=255)
gATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:394747/45‑1 (MQ=255)
tGAAAGTTAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2266225/41‑1 (MQ=255)
tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:3255499/41‑1 (MQ=255)
tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:930618/69‑1 (MQ=255)
tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:3224477/69‑1 (MQ=255)
tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:573378/69‑1 (MQ=255)
gAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:3106256/68‑1 (MQ=255)
gAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:3086634/68‑1 (MQ=255)
aGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCt < 1:1686863/68‑1 (MQ=255)
aCGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAg > 1:3007300/1‑68 (MQ=255)
aCGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAg > 1:1338400/1‑68 (MQ=255)
cGGCGTCCATCTTTCTGATGAGGAAGGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAgg > 1:1474800/1‑68 (MQ=255)
cGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATTCAgg > 1:122002/1‑68 (MQ=255)
cGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAgg > 1:1995739/1‑68 (MQ=255)
cGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAgg > 1:1236708/1‑68 (MQ=255)
gCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGaa < 1:2726659/68‑1 (MQ=255)
tCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAAc > 1:840798/1‑68 (MQ=255)
tCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACg > 1:1343292/1‑69 (MQ=255)
cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:3333019/1‑69 (MQ=255)
cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:288099/1‑69 (MQ=255)
cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:445025/1‑69 (MQ=255)
cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:2185535/1‑69 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGTTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:2409344/1‑68 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:802418/1‑67 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:1485925/1‑68 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:2772686/1‑68 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:1658932/1‑68 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:1888167/1‑68 (MQ=255)
tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:307662/1‑68 (MQ=255)
cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:1522807/1‑69 (MQ=255)
cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:2365187/1‑69 (MQ=255)
cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:2236664/1‑69 (MQ=255)
cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:1893693/1‑69 (MQ=255)
cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:2100424/1‑69 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2547270/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:774549/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:1879509/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:316880/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2519207/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:3040980/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2849114/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2347926/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2903541/1‑59 (MQ=255)
tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGAATTTTGGt > 1:1559450/1‑41 (MQ=37)
tttCTGATGAGCAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2086869/1‑59 (MQ=255)
tgatgaAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:1637531/39‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1870099/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1718305/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1700660/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2966101/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1581362/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2158215/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1523948/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:197341/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2472251/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2631683/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2140985/69‑1 (MQ=255)
aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:948341/69‑1 (MQ=255)
|
CACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTGCATGAAAAACAGAT > minE/1699011‑1699142
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
