Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,709,926 |
C→T |
58.0% |
V374V (GTG→GTA) |
yphE ← |
fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,709,926 | 0 | C | T | 58.0%
| 12.1
/ 35.2
| 50 | V374V (GTG→GTA) | yphE | fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (10/11); new base T (4/25); total (14/36) |
| Fisher's exact test for biased strand distribution p-value = 1.20e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 |
GGCGGCCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAG > minE/1709860‑1709989
|
ggcggcCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGcac > 1:2918925/1‑69 (MQ=255)
ggcggcCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGcac > 1:2100635/1‑69 (MQ=255)
cGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGAttttt > 1:357541/1‑69 (MQ=255)
cGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGAttttt > 1:51427/1‑69 (MQ=255)
tCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:3426528/69‑1 (MQ=255)
tCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:686193/69‑1 (MQ=255)
tCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:178102/69‑1 (MQ=255)
gcAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:2742563/68‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCTCTGTATTTTc < 1:2746740/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:546850/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:569935/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:387618/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:667649/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:68513/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:848997/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3402144/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3360576/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3282732/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:857140/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3127525/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:906712/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:106787/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1547169/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1403462/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1567765/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1026898/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1617201/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1738129/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:215548/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:2622356/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1221838/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1890989/69‑1 (MQ=255)
gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1802857/69‑1 (MQ=255)
ggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc > 1:2491560/1‑67 (MQ=255)
ggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCg > 1:2817895/1‑68 (MQ=255)
ggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCg > 1:3237295/1‑68 (MQ=255)
ggATGGTGGACAATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTTTATTTTCg > 1:2626921/1‑68 (MQ=255)
tggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa < 1:1137529/69‑1 (MQ=255)
tggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa < 1:3075553/69‑1 (MQ=255)
tggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa < 1:222351/69‑1 (MQ=255)
ggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:542815/68‑1 (MQ=255)
ggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:2343315/68‑1 (MQ=255)
ggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:2643188/68‑1 (MQ=255)
gACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:1777174/67‑1 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:611949/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:1430354/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:551409/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:1961006/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:1180070/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:2035143/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:2915095/1‑68 (MQ=255)
tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:970839/1‑68 (MQ=255)
gcagcaCACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAg > 1:875074/1‑68 (MQ=255)
|
GGCGGCCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAG > minE/1709860‑1709989
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
