Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I0 R1	4577	57.4	3436498	77.7%	2670158	63.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,725,308	G→A	45.4%	F974F (TTC→TTT)	purL ←	phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,725,308	0	G	A	45.4%	11.6 / 51.3	66	F974F (TTC→TTT)	purL	phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand): ref base G (22/14); new base A (10/20); total (32/34)
Fisher's exact test for biased strand distribution p-value = 2.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

GCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATA  >  minE/1725244‑1725370

gCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATa                                                            <  1:3336221/69‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCTGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:950524/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1077318/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:2186005/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:2182138/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:800937/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:755600/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:725972/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:2489550/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:2711690/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1702903/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1698302/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1686813/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1530584/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1178656/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:618505/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:352031/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:905081/61‑1 (MQ=255)

                       gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1240615/61‑1 (MQ=255)

                        tttCTGCCCACGAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:1271349/60‑1 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:2413421/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:2216990/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:2255742/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:1975857/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:458087/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:2664189/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:3392123/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:1434697/1‑68 (MQ=255)

                          tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:940311/1‑68 (MQ=255)

                          tCTGCCCACCAGACAAGCAACGTGGTGAGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac                                   >  1:2909166/1‑68 (MQ=255)

                           cTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg                                             <  1:791996/57‑1 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGGCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc                 >  1:3391653/1‑69 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGt                           >  1:1321517/1‑59 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGaaa                  >  1:620044/1‑68 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc                 >  1:1954678/1‑69 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc                 >  1:2420927/1‑69 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc                 >  1:2230933/1‑69 (MQ=255)

                                           cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc                 >  1:1708444/1‑69 (MQ=255)

                                                 ggtgCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTg           <  1:2590610/69‑1 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:1735668/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:577046/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:1284111/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:1312969/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:1707812/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:2763208/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:264213/1‑69 (MQ=255)

                                                    gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc        >  1:2614221/1‑69 (MQ=255)

                                                       gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa      >  1:1415498/1‑68 (MQ=255)

                                                       gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa      >  1:2446596/1‑68 (MQ=255)

                                                       gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa      >  1:1825012/1‑68 (MQ=255)

                                                       gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa      >  1:1836752/1‑68 (MQ=255)

                                                       gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa      >  1:1217625/1‑68 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:812005/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:1118461/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:583151/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:3168886/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:284872/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:2337114/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:2084180/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:1974691/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:1784307/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:152130/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt   <  1:1512388/69‑1 (MQ=255)

                                                         tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTCCCCTACAt   <  1:954872/69‑1 (MQ=255)

                                                          ttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATa  >  1:2389325/1‑69 (MQ=255)

                                                          ttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATa  >  1:1372787/1‑69 (MQ=255)

GCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATA  >  minE/1725244‑1725370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A