Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I0 R1
|
4577 |
57.4 |
3436498 |
77.7% |
2670158 |
63.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,727,756 |
A→G |
46.4% |
V158V (GTT→GTC) |
purL ← |
phosphoribosylformyl‑glycineamide synthetase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,727,756 | 0 | A | G | 46.4%
| ‑0.8
/ 32.7
| 41 | V158V (GTT→GTC) | purL | phosphoribosylformyl‑glycineamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (13/9); new base G (3/16); total (16/25) |
| Fisher's exact test for biased strand distribution p-value = 9.25e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 |
AGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCAT > minE/1727692‑1727824
|
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:101536/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:2687414/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:2881304/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:3073610/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:200277/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:3368557/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:1638554/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:395604/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:14080/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:1398471/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:1340355/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:1312948/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:788299/66‑1 (MQ=255)
ggccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCCGCCCCAGCAAATCTACGCTGGTGAc < 1:3005027/66‑1 (MQ=255)
gccaagACGCAGGTTAGCGTCAATTAGCGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:3208896/66‑1 (MQ=255)
gCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGtt < 1:3388933/69‑1 (MQ=255)
cAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGtt < 1:2194700/68‑1 (MQ=255)
aGGTTAGCGTCAATTAGAGCCTGACGGCCCTGCCCCAGCAAATCTACGCTGGTGAc < 1:1227277/56‑1 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:772448/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:3355250/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:83341/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:2621650/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:1580735/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:2236198/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:2104785/1‑69 (MQ=255)
tCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCa > 1:1233591/1‑69 (MQ=255)
gCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTg < 1:231309/55‑1 (MQ=255)
gCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTg < 1:694959/55‑1 (MQ=255)
gCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTg < 1:1719437/55‑1 (MQ=255)
gCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTg < 1:1356538/55‑1 (MQ=255)
gCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTg < 1:2036553/55‑1 (MQ=255)
gCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTg < 1:2778854/55‑1 (MQ=255)
cAAATCTACGCTGGTGACCGGAGTCGGTTGATGGTGTGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:3030001/1‑68 (MQ=255)
cAAATCTACGCTGGTGACCGGAGTCGGTTGATGGTGTGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:3026406/1‑68 (MQ=255)
cAAATCTACGCTGGTGACCGGAGTCGGTTGATGGTGTGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:1594409/1‑68 (MQ=255)
aaaTCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTg > 1:2578648/1‑52 (MQ=255)
aaaTCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:2845279/1‑67 (MQ=255)
aaaTCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:2740670/1‑67 (MQ=255)
aaaTCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:2254551/1‑67 (MQ=255)
aaaTCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCaa > 1:465013/1‑67 (MQ=255)
aTCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCaaaaaa < 1:558475/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCGAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:1734617/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:1078344/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:501575/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:362829/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:3289500/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:3071167/69‑1 (MQ=255)
aaCCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCcat < 1:2213500/69‑1 (MQ=255)
|
AGCCAAGACGCAGGTTAGCGTCGATCAGCGCCTGACGGCCCTGCCCCAGCAAATCAACGCTGGTAACCGGAGTCGGTTGATGGTGGGCAAACAACTGCTCTGCATCATCTAAAGCAAAAAAGACCGTTTCCAT > minE/1727692‑1727824
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
