Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I0 R1 4577 57.4 3436498 77.7% 2670158 63.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,781,070 C→T 45.4% A166A (GCC→GCT)  ypjD → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,781,0700CT45.4% 19.5 / 80.9 99A166A (GCC→GCT) ypjDpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (21/33);  new base T (8/37);  total (29/70)
Fisher's exact test for biased strand distribution p-value = 2.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.34e-01

Only 100 of 106 total aligned reads displayed.

GCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAAC  >  minE/1781005‑1781136
                                                                 |                                                                  
gCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCgccgcc                                                                 >  1:1009646/1‑69 (MQ=255)
gCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCgccgcc                                                                 >  1:1114685/1‑69 (MQ=255)
gCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCgccgcc                                                                 >  1:601106/1‑69 (MQ=255)
gCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCgccgcc                                                                 >  1:404393/1‑69 (MQ=255)
   aCGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTg                                                              <  1:3201159/69‑1 (MQ=255)
   aCGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTg                                                              <  1:3018435/69‑1 (MQ=255)
             tGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGc                                                    <  1:2869436/69‑1 (MQ=255)
             tGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGc                                                    <  1:2026113/69‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:1879720/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:3179680/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2022293/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2094875/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2212314/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2263502/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2400669/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2440929/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2559698/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2578881/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:303843/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2831551/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2835343/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2871720/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:1638810/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:1199458/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:850913/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTTTTTTCCTATACCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:935277/68‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTTTACGCGCTGCa                                                   <  1:3428333/69‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   <  1:307785/69‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   <  1:303466/69‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   <  1:2298001/69‑1 (MQ=255)
              gCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   <  1:2296235/69‑1 (MQ=255)
               cTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCaa                                                  >  1:1827503/1‑69 (MQ=255)
               cTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCaa                                                  >  1:1858716/1‑69 (MQ=255)
               cTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCaa                                                  >  1:3343976/1‑69 (MQ=255)
               cTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCaa                                                  >  1:3099513/1‑69 (MQ=255)
                  gTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   >  1:75750/1‑65 (MQ=255)
                  gTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   >  1:79737/1‑65 (MQ=255)
                  gTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   >  1:390671/1‑65 (MQ=255)
                  gTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   >  1:343673/1‑65 (MQ=255)
                  gTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   >  1:1125380/1‑65 (MQ=255)
                  gTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCa                                                   >  1:341733/1‑65 (MQ=255)
                   tGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTgg                                              >  1:3180076/1‑69 (MQ=255)
                   tGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTgg                                              >  1:3043508/1‑69 (MQ=255)
                           ggCTTATCGCTTTTTTCCTATGCCACGCTAATTATCGCTGCCCTGTACGCGCTGc                                                    <  1:2300678/55‑1 (MQ=255)
                               tATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGgattgatt                                  <  1:2877588/69‑1 (MQ=255)
                                  cGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTa                                 <  1:2932526/67‑1 (MQ=255)
                                  cGCTCTTTTACTATGCCACGCTAATTATCGCCGCCCTCTACGCGCTGCAACTGGCGTGGATTGATTa                                 <  1:863916/67‑1 (MQ=255)
                                   gCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTAcc                               <  1:3298707/68‑1 (MQ=255)
                                              ataccACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGAATACCAACt                           >  1:2583736/4‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:620296/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:246940/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:228820/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:2731580/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:2268057/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:2234568/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACt                           >  1:2891938/1‑61 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:608267/69‑1 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:1050643/69‑1 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:2985197/69‑1 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:3259993/69‑1 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:1417811/69‑1 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:2512347/69‑1 (MQ=255)
                                              aTGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                   <  1:1708105/69‑1 (MQ=255)
                                                 ccACGCTATTTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:2667744/68‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaaga                <  1:1376427/69‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaaga                <  1:1480328/69‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaaga                <  1:2072796/69‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:299339/68‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:711691/68‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:1621847/68‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:2015531/68‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:3161594/68‑1 (MQ=255)
                                                 ccACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaag                 <  1:2166345/68‑1 (MQ=255)
                                                  cACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaaga                <  1:2582531/68‑1 (MQ=255)
                                                     gCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCt            <  1:3158721/69‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:2601873/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:760612/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1460818/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:74429/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:2553691/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1492387/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:848288/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1368707/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1571742/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:358228/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:170803/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:3411476/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1776440/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1807550/67‑1 (MQ=255)
                                                           tATCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1900028/67‑1 (MQ=255)
                                                            atagccgccCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGTCGttt     >  1:21400/4‑69 (MQ=255)
                                                            aTCGCTGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg        <  1:1584189/66‑1 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGGAACAGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTgg          >  1:1264544/1‑64 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGttt     >  1:806533/1‑69 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGttt     >  1:846120/1‑69 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGttt     >  1:1495733/1‑69 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGttt     >  1:2885707/1‑69 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGttt     >  1:907542/1‑69 (MQ=255)
                                                            aTCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGttt     >  1:3236097/1‑69 (MQ=255)
                                                               gccgccCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAAc  <  1:532665/69‑1 (MQ=255)
                                                                 |                                                                  
GCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAAC  >  minE/1781005‑1781136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A